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    Isoleucine-requiring Nicotiana plant deficient in threonine deaminase

    Well characterized auxotrophic mutants would greatly assist the genetic manipulation of flowering plants, but such mutants are rare1–3. Moreover, auxotrophic mutants isolated at the plant level4–8 and some of tho...

    Vladimir Sidorov, László Menczel, Pál Maliga in Nature (1981)