C. elegans
Methods and Applications
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Article
Open AccessInaugural Charles River World Congress on Animal Models in Drug Discovery and Development
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Article
Primary culture of Caenorhabditis elegans develo** embryo cells for electrophysiological, cell biological and molecular studies
Cell culture is an invaluable tool for investigation of basic biological processes. However, technical hurdles including low cell yield, poor cell differentiation and poor attachment to the growth substrate ha...
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Book
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Isolation of C. elegans deletion mutants following ENU mutagenesis and thermostable restriction enzyme PCR screening
The ability to generate null mutants is essential for studying gene function. Gene knockouts in Caenorhabditis elegans can be generated in a high throughput manner using chemical mutagenesis followed by polymeras...
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Protocol
In Vitro Culture of C. elegans Somatic Cells
Because of technical hurdles, large-scale cell culture methods have not been widely exploited until recently for the study of Caenorhabditis elegans. Culturing differentiated cells from larvae and adult worms is ...
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Protocol
An Overview of C. elegans Biology
The establishment of Caenorhabditis elegans as a “model organism” began with the efforts of Sydney Brenner in the early 1960s. Brenner’s focus was to find a suitable animal model in which the tools of genetic ana...
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Article
Maintenance of cell volume in the central nervous system
Maintenance of the ionic and osmotic composition and volume of intra- and extracellular fluids in the brain is crucial for normal functioning of the central nervous system (CNS). Osmoregulation in the CNS is m...
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Chapter
Optical Methods for Quantifying Cell Volume Changes in Isolated Nephron Segments
Animal cell volume is determined by extracellular and intracellular solute content. Measurements of cell volume changes induced by osmotic, ionic, electrophysiological and/or pharmacological manipulations can ...
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Chapter
Volume Regulation in the Collecting Duct and Related Epithelia
Under normal physiological conditions, cells of tight, Na+-reabsorbing urinary epithelia, such as the collecting tubule, are exposed to both anisosmotic and isosmotic volume stress. A common but poorly understood...
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Article
Ouabain-induced cell swelling in rabbit cortical collecting tubule: NaCl transport by principal cells
Ouabain had no effect on the volume of intercalated cells of DOCA-stimulated rabbit cortical collecting tubules, but caused principal cells to swell rapidly at an initial rate of 67% min., Principal cells swel...
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Article
Cell membrane water permeability of rabbit cortical collecting duct
The water permeability (P osm) of the cell membranes of isolated perfused rabbit cortical collecting ducts was measured by quantitative light microscopy. Water permeability of the basolateral membrane, corrected ...
