![Loading...](https://link.springer.com/static/c4a417b97a76cc2980e3c25e2271af3129e08bbe/images/pdf-preview/spacer.gif)
-
Protocol
DNA Extraction from Natural Environments
Until recently, studies on microbial communities present in natural environments relied on conventional optical microscopic observation and cultivation-based approaches. Although these traditional approaches r...
-
Protocol
Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments
There has been a growing acknowledgment of both the ecological and biotechnological importance of microbes in natural environments. Concerns about the nonrepresentative nature of traditional analytical methods...
-
Article
Terminal restriction fragment length polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments
Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR pro...
-
Article
Open AccessBacterial activity in cystic fibrosis lung infections
Chronic lung infections are the primary cause of morbidity and mortality in Cystic Fibrosis (CF) patients. Recent molecular biological based studies have identified a surprisingly wide range of hitherto unrepo...
-
Article
Chromosomal genes conferring tolerance to heavy metal (Ag) toxicity
Bacterial strains were isolated from sediment samples from the Thames River. Successive transfer growth of the various strains on nutrient agar containing increasing concentrations of AgNO3 revealed that three of...
-
Article
Open AccessStudying bacteria in respiratory specimens by using conventional and molecular microbiological approaches
Drawing from previous studies, the traditional routine diagnostic microbiology evaluation of samples from chronic respiratory conditions may provide an incomplete picture of the bacteria present in airways dis...
-
Article
Molecular Microbiological Characterization of Preterm Neonates at Risk of Bronchopulmonary Dysplasia
The role of infection in bronchopulmonary dysplasia (BPD) is unknown. We present an observational study of 55 premature infants born weighing less than 1.3 kg within two level III neonatal intensive care units...
-
Article
Determining the Effects of a Spatially Heterogeneous Selection Pressure on Bacterial Population Structure at the Sub-millimetre Scale
A key interest of microbial ecology is to understand the role of environmental heterogeneity in sha** bacterial diversity and fitness. However, quantifying relevant selection pressures and their effects is c...
-
Article
Next-Generation Sequencing in the Analysis of Human Microbiota
The development of next-generation sequencing (NGS) presents an unprecedented opportunity to investigate the complex microbial communities that are associated with the human body. It offers for the first time ...
-
Article
Cyclic antimicrobial R-, W-rich peptides: the role of peptide structure and E. coli outer and inner membranes in activity and the mode of action
This study compares the effect of cyclic R-, W-rich peptides with variations in amino acid sequences and sizes from 5 to 12 residues upon Gram negative and Gram positive bacteria as well as outer membrane-defi...
-
Article
A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation
Chronic polymicrobial lung infections in adult cystic fibrosis patients are typically dominated by high levels of Pseudomonas aeruginosa. Determining the impact of P. aeruginosa growth on airway secretion composi...
-
Article
Open AccessContributions of Composition and Interactions to Bacterial Respiration Are Reliant on the Phylogenetic Similarity of the Measured Community
Bacterial diversity underpins many ecosystem functions; however, the impact of within-species variation on the relationship between diversity and function remains unclear. Processes involving strain differenti...
-
Article
Open AccessLung function and microbiota diversity in cystic fibrosis
Chronic infection and concomitant airway inflammation is the leading cause of morbidity and mortality for people living with cystic fibrosis (CF). Although chronic infection in CF is undeniably polymicrobial, ...