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Article
Open AccessHalf a century of the reverse transcriptase—happy birthday!
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Chapter
Phages as Therapy or “Dietary Supplements” Against Multiresistant Bacteria?
Bacteria and phages form an ecosystem and play a role in obesity, in Intestinal bowel disease, neurological disorders, in the brain-gut axis and more recently in anticancer therapies. We have shown that fecal ...
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Article
On the occasion of the retirement of Marc Regenmortel as Editor-in-Chief of Archives of Virology
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Article
Open AccessThe intron-enriched HERV-K(HML-10) family suppresses apoptosis, an indicator of malignant transformation
Human endogenous retroviruses (HERVs) constitute 8% of the human genome and contribute substantially to the transcriptome. HERVs have been shown to generate RNAs that modulate host gene expression. However, ex...
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Article
Open AccessAbolishing HIV-1 infectivity using a polypurine tract-specific G-quadruplex-forming oligonucleotide
HIV is primarily transmitted by sexual intercourse and predominantly infects people in Third World countries. Here an important medical need is self-protection for women, particularly in societies where condom...
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Protocol
RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential...
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Article
Open AccessWhat contemporary viruses tell us about evolution: a personal view
Recent advances in information about viruses have revealed novel and surprising properties such as viral sequences in the genomes of various organisms, unexpected amounts of viruses and phages in the biosphere...
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Article
Open AccessShort hairpin-looped oligodeoxynucleotides reduce hepatitis C virus replication
Persistent infection with hepatitis C virus (HCV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Standard therapy consists of a combination of interferon-alpha and riba...
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Article
Open AccessDestruction HIV viral RNA by siDNA triggering RNAse H
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Article
Open AccessEvolution of viruses and antiviral defense
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Article
Open AccessSilencing of viral RNAs by small double-stranded siDNA
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Article
Open AccessShort hairpin-loop-structured oligodeoxynucleotides reduce HSV-1 replication
The Herpes simplex virus (HSV) is known as an infectious agent and widespread in the human population. The symptoms of HSV infections can range from mild to life threatening, especially in immune-compromised i...
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Article
Inhibition of influenza A virus replication by short double-stranded oligodeoxynucleotides
Influenza A virus causes prevalent respiratory tract infections in humans. Small interfering RNA (siRNA) and antisense oligonucleotides (asODNs) have been used previously for silencing the RNA genome of influe...
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Article
RNase H-mediated retrovirus destruction in vivo triggered by oligodeoxynucleotides
The HIV-1 RNase H can be prematurely activated by oligodeoxynucleotides targeting the highly conserved polypurine tract required for second strand DNA synthesis1,2,3,4,5. This inhibits retroviral replication in c...
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Article
Antibodies from a DNA peptide vaccination decrease the brain amyloid burden in a mouse model of Alzheimer’s disease
The neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation of amyloid peptide Aβ in the brain derived from proteolytic cleavage of the amyloid precursor protein (APP). Vaccination of m...
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Article
A combination of plasmid DNAs encoding murine fetal liver kinase 1 extracellular domain, murine interleukin-12, and murine interferon-γ inducible protein-10 leads to tumor regression and survival in melanoma-bearing mice
The vascular endothelial growth factor (VEGF) and its interaction with the vascular endothelial growth factor receptor 2 [VEGFR2/murine fetal liver kinase 1 (Flk-1), human kinase domain receptor] are an import...
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Article
Linear closed mini DNA generated by the prokaryotic cleaving-joining enzyme TelN is functional in mammalian cells
For application of DNA in gene medicine plasmid or viral DNA is usually used as a vector for the gene of interest. To generate DNA with a minimum of foreign DNA sequences, we used the prokaryotic telomerase, ...
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Article
Synergistic effect of a combined DNA and peptide vaccine against gp100 in a malignant melanoma mouse model
Vaccination against tumors relies on tumor-associated antigens, and has been quite successful with synthetic peptides used as immunogens. Gp100 is a human melanoma-associated antigen (hgp100) with a highly hom...
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Article
Tumor regression induced by intratumoral injection of DNA coding for human interleukin 12 into melanoma metastases in gray horses
Preclinical studies investigating new therapeutic principles against melanoma are presently being carried out in mouse models; however, these are not optimal. Here we describe a novel animal model using gray h...
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Article
Induction of long-lasting cytokine effect by injection of IL-12 encoding plasmid DNA
We have recently demonstrated that DNA coding for both subunits of the murine IL-12 heterodimer exhibits a strong antimetastatic effect against B16-melanoma in C57BL/6 mice and after intratumoral injection tum...
