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Article
Open AccessSensory nerve conduction stimulus threshold measurements of the infraorbital nerve and its applicability as a diagnostic tool in horses with trigeminal-mediated headshaking
To determine whether sensory nerve conduction stimulus threshold measurements of the infraorbital nerve are able to differentiate horses with idiopathic trigeminal-mediated headshaking (i-TMHS) from healthy ho...
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Article
Open AccessExperimental cross-species infection of donkeys with equine hepacivirus and analysis of host immune signatures
The Equine Hepacivirus (EqHV) is an equine-specific and liver-tropic virus belonging to the diverse genus of Hepaciviruses. It was recently found in a large donkey (Equus asinus) cohort with a similar seroprevale...
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Article
Open AccessMetabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet
Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could prov...
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Article
Open AccessWeight loss is linearly associated with a reduction of the insulin response to an oral glucose test in Icelandic horses
Insulin dysregulation (ID) goes along with lasting or transient hyperinsulinemia able to trigger equine laminitis, a painful and crippling foot condition. Promoting weight loss through dietary changes and phys...
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Article
Open AccessBetulinic acid shows anticancer activity against equine melanoma cells and permeates isolated equine skin in vitro
Equine malignant melanoma (EMM) is a frequently occurring dermoepidermal tumor in grey horses. Currently available therapies are either challenging or inefficient. Betulinic acid (BA), a naturally occurring tr...
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Article
Open AccessLower plasma trans-4-hydroxyproline and methionine sulfoxide levels are associated with insulin dysregulation in horses
Insulin dysregulation in horses is a metabolic condition defined by high insulin concentrations in the blood and peripheral insulin resistance. This hyperinsulinemia is often associated with severe damage in t...
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Article
Open AccessRetrospective analysis of insulin responses to standard dosed oral glucose tests (OGTs) via naso-gastric tubing towards definition of an objective cut-off value
Insulin dysregulation (ID) with basal or postprandial hyperinsulinemia is one of the key findings in horses and ponies suffering from the equine metabolic syndrome (EMS). Assessment of ID can easily be perform...
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Article
Open AccessGermline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family
The bulldog calf syndrome is a lethal form of the inherited congenital chondrodysplasias. Among the progeny of the polled Holstein bull Energy P cases of lethal chondrodysplasia were observed. Pedigrees of the...
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Article
Open AccessAcute and chronic infections with nonprimate hepacivirus in young horses
The recently discovered nonprimate hepacivirus (NPHV) naturally infects horses and is the closest known homolog of hepatitis C virus to date. Within a follow-up study acute field infections were monitored in f...
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Article
Open AccessPhenotypic classification of variability of non-syndromic congenital cleft lip and jaw in Vorderwald × Montbéliarde cattle
Non-syndromic congenital cleft lip and jaw (CLJ) is a condition reported in several cattle breeds, but a detailed classification system does not exist for cattle. The objective of the present study was to desc...
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Article
Open AccessLocal and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma
Equine melanoma has a high incidence in grey horses. Xenogenic DNA vaccination may represent a promising therapeutic approach against equine melanoma as it successfully induced an immunological response in oth...
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Article
Open AccessLocal and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma
Equine melanoma has a high incidence in grey horses. Xenogenic DNA vaccination may represent a promising therapeutic approach against equine melanoma as it successfully induced an immunological response in oth...
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Article
Open AccessPharmacokinetics and thrombolytic effects of the recombinant tissue-type plasminogen activator in horses
To test the efficacy of the recombinant tissue-type plasminogen activator (rt-PA) alteplase in horses, the thrombolytic effect was tested in in vitro generated equine thrombi. The extent of lysis was determined b...
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Article
Open AccessMorphometric magnetic resonance imaging and genetic testing in cerebellar abiotrophy in Arabian horses
Cerebellar abiotrophy (CA) is a rare but significant disease in Arabian horses caused by progressive death of the Purkinje cells resulting in cerebellar ataxia characterized by a typical head tremor, jerky hea...
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Article
Open AccessComparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity
Reproducibly high transfection rates with low methodology-induced cytotoxic side effects are essential to attain the required effect on targeted cells when exogenous DNA is transfected. Different approaches an...
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Article
Isolation, establishment, and characterization of ex vivo equine melanoma cell cultures
Gray horses spontaneously develop metastatic melanomas that resemble human disease, and this is often accompanied with metastasis to other organs. Unlike in other species, the establishment of primary equine m...
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Article
Open AccessAssessment of Equine Autoimmune Thrombocytopenia (EAT) by flow cytometry
Thrombocytopenia is a platelet associated process that occurs in human and animals as result of i) decreased production; ii) increased utilization; iii) increased destruction coupled to the presence of antibod...
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Article
Tumor regression induced by intratumoral injection of DNA coding for human interleukin 12 into melanoma metastases in gray horses
Preclinical studies investigating new therapeutic principles against melanoma are presently being carried out in mouse models; however, these are not optimal. Here we describe a novel animal model using gray h...
