-
Article
An X-ray microprobe facility using synchrotron radiation
An X-ray microprobe for trace elemental analysis at micrometer spatial resolutions, using synchrotron radiation (SR), is under development. The facility consists of two beamlines, one including a 1∶1 focusing ...
-
Chapter
An X-Ray Microprobe Facility Using Synchrotron Radiation
An X-ray microprobe for trace elemental analysis at micrometer spatial resolutions, using synchrotron radiation (SR), is under development. The facility consists of two beamlines, one including a 1:1 focusing ...
-
Article
Restriction fragment polymorphism in the sex-determining region of the Y chromosomal DNA of European wild mice
Using 32P-labeled probe consisting mainly of (GATA)n we have shown that a male specific Alu1 DNA blot pattern which defines the Y chromosome sex-determining locus in inbred mice is highly polymorphic in wild mice...
-
Article
Studies of aluminum in rat brain
The effects of high aluminum concentrations in rat brain were studied using14C autoradiography to measure the uptake of [14C]2deoxy-d-glucose ([14C]2dG) and microbeam proteon-induced X-ray emission (microPIXE) wi...
-
Article
Localization of vanadium-containing particles in the lungs of uranium/vanadium miners
Several geological formations of the Utah-Colorado mining region mined for uranium ore during and after World War II had been mined earlier for vanadium. Therefore, most miners and millers from that region wer...
-
Article
A DNA sequence which shows genomic variation in a, alpha and HO strains of Saccharomyces cerevisiae
HindIII digested DNA from various mutant strains of Saccharomyces cerevisiae probed with a 340 by nucleotide sequence in M13mp8 derived from a mouse liver cDNA clone p1581 showed strong hybridization to a 4.1 kb ...
-
Article
Role of nuclear analytical probe techniques in biological trace-element research
Many biomedical experiments require the qualitative and quantitative localization of trace elements with high sensitivity and good spatial resolution. The feasibility of measuring the chemical form of the elem...
-
Article
Some uncertainties associated with preparation of standards in organic matrix
Until recently no techniques which have multielement detection capability, high sensitivity and good spatial resolution in relatively thick tissue section have existed. The use of proton induced x-ray emission...
-
Article
Sex chromosome associated satellite DNA: Evolution and conservation
Satellites visible in female but not in male DNA were isolated from the snakesElaphe radiata (satellite IV, p = 1.708 g · cm−3) andBungarus fasciatus (BK1 minor, p=1.709 g · cm−3). The satellites cross hybridize....
-
Article
Improved method for detection of cellular transcripts by in situ hybridization: Detection of poly (A) sequences in individual cells
A method for detecting RNA base sequences in individual cells by in situ hybridization with radioactive probes and autoradiography was developed. Cells are fixed with glutaraldehyde at low concentration and de...
-
Article
Sex specific chromosome polymorphisms in the common Indian Krait, Bungarus caeruleus Schneider (Ophidia, Elapidae)
Chromosome analyses of common Indian Krait, B. caeuleus from three geographical regions of India have revealed variable diploid numbers of 43, 44 and 45 in different female individuals but a constant diploid numb...
-
Article
Conservation and chromosomal localization of DNA satellites in balenopterid whales
DNA satellites were isolated from three balenopterid species, viz. the minke, sei, and fin whales. In each of them at least two DNA satellites were recognizable with buoyant densities in neutral CsCl of ρ=1.70...
-
Article
Effect of different denaturing agents on the detectability of specific DNA sequences of various base compositions by in situ hybridisation
In situ hybridisation of certain AT rich and GC rich satellite DNA complementary RNAs (cRNAs) to their homologous chromosomes at their respective optimal rate temperatures (TOPTS) after denaturation with various ...
-
Article
The chromosomal localisation of satellite DNA in Ptyas mucosus (Ophidia, Colubridae)
Ptyas mucosus male DNA has a repetitious DNA satellite (p= 1.700 g cm−3) constituting 5% of the haploid genome. In situ hybridisation of radioactive complementary RNA (cRNA) has revealed that satellite sequences...
-
Article
Satellite DNA and evolution of sex chromosomes
The satellite DNA (satellite III) which is mainly represented in the female of Elaphe radiata (Ophidia, Colubridae) has been isolated and its buoyant density has been determined (ϱ=1.700 g cm−3). In situ hybridis...
-
Article
Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation
Hybridising certain AT-rich satellite complementary RNAs (cRNAs) to their homologous chromosomal DNA sequences at different temperatures of incubation results in a different dispersion of autoradiographic labe...
-
Article
The chromosomal localisation of human satellite DNA I
The major concentrations of human satellite DNA I (1.688 g/ml) have been localised on human chromosome preparations by the technique of in situ hybridisation using radioactive complementary RNA synthesised in vit...
-
Article
The chromosomal location of human satellite DNA III
In situ hybridisation of radioactive complementary RNA has been used to localise the chromosomal distribution of human satellite DNA III. This DNA is found to be concentrated in paracentromeric heterochromatin m...
-
Article
Complex formation between Poly-r (U) and various chromosomal loci in Rhynchosciara
Polyribouridylic acid [poly(U)] labelled with tritium was annealed to chromosomes from Rhynchosciara salivary glands to detect the distribution of A-T rich DNA. A clear pattern of binding was observed to several ...