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Chapter and Conference Paper
Extraction of the non-equilibrium energy spectrum in high Reynolds number turbulence
The structure of the energy spectrum in the inertial subrange is studied using the DNS data for turbulence in a periodic box at high Reynolds numbers. The Kolmogorov spectrum,
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Article
Effects of inorganic phosphate on cross-bridge behavior after photorelease of ATP in permeabilized cells of rat skeletal muscle
Effects of 20 mM inorganic phosphate on the cross-bridge behavior after photorelease of ATP from caged ATP was studied by X-ray diffraction in rat skinned psoas muscle fibers at 16°C. In the first 30 ms after...
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Article
Single turnover of cross-bridge ATPase in rat muscle fibers studied by photolysis of caged ATP
A mechanical study on skinned rat psoas muscle fibers was performed at about 16°C with X-ray diffraction and caged-ATP photolysis. The amount of photoreleased ATP was set <0.2 mM for analysis of a `single turn...
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Article
Effects of ADP and low ATP on the Ca2+-sensitive transient contraction upon photolysis of caged ATPin rat muscle fibres: a study on the Bremel–Webertype cooperation
Caged ATP was photolysed in rat psoas fibres under various conditions to examine whether ADP plays a special role in the␣‘Bremel–Weber type cooperation’, viz. the Ca2+-like action of the rigor cross-bridges. V...
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Article
Mechanical response to photolytic ATP pulses of skinned muscle fibres pre-activated with a small pulse of ATP
(1) Skinned fibres from rat psoas muscle were placed in oil and activated at 10°C by pulse photolysis of caged ATP. From the force and stiffness transients on small ATP pulses, we estimated the concentration a...
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Chapter
Effects of Ionic Strength on Force Transients Induced by Flash Photolysis of Caged ATP in Covalently Crosslinked Rabbit Psoas Muscle Fibers
Single fibers from glycerinated rabbit psoas muscle were treated with 1-ethyl-3[3-(dimethylamino) propyl] carbodiimide (EDC), after rigor was induced, to crosslink myosin heads to actin. The optimally pre-stre...
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Article
Time course of rise of muscle stiffness at onset of contraction induced by photorelease of ATP
Isometric contraction (9–10°C) of skinned fibres from rat psoas muscle was elicited by photorelease of ATP (1.3–1.4 mM), and force (F), in-phase (I) and quadrature (Q) stiffness were monitored. The sinusoidal len...
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Article
Effects of ethylene glycol and calcium on the kinetics of contraction induced by photo-release of low concentrations of ATP in rat psoas muscle fibres
To induce isometric contraction in the absence of Ca2+ (10mm EGTA), low concentrations (130 μM) of ATP were photoreleased from caged ATP in skinned fibres from rat psoas muscle at 15–16° C. The magnitude of contr...
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Article
Effects of ethylene glycol on the kinetics of contraction on flash photolysis of caged ATP in rat psoas muscle fibres
ATP (1–1.2mm) was photoreleased from caged ATP (5mm) in skinned fibres from rat psoas muscle at 15–17° C, to examine the effects of ethylene glycol (EG; 20% in solvent) on the kinetics of isometric contraction. M...
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Article
Effects of 2,3-butanedione monoxime on contraction of frog skeletal muscles: An X-ray diffraction study
We studied the effects of BDM (2,3-butanedione monoxime) on the tetanic contraction of frog skeletal muscles using an X-ray diffraction technique. BDM significantly increased the resting equatorial intensity r...
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Article
Mechanism of action of 2, 3-butanedione 2-monoxime on contraction of frog skeletal muscle fibres
The mechanism of the inhibitory effect of 2, 3-butanedione 2-monoxime (BDM) on contraction of frog skeletal muscles was studied using skinned fibres and aequorin-injected intact fibres. The tension development...
