44 Result(s)
within
Johannes Messinger
English
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Article
Open AccessOn the simulation and interpretation of substrate-water exchange experiments in photosynthetic water oxidation
Water oxidation by photosystem II (PSII) sustains most life on Earth, but the molecular mechanism of this unique process remains controversial. The ongoing identification of the binding sites and modes of the ...
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Article
Open AccessAuthor Correction: Structural evidence for intermediates during O2 formation in photosystem II
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Protocol
Measurements of Oxygen Evolution in Photosynthesis
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We...
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Article
Open AccessEvolutionary diversity of proton and water channels on the oxidizing side of photosystem II and their relevance to function
One of the reasons for the high efficiency and selectivity of biological catalysts arise from their ability to control the pathways of substrates and products using protein channels, and by modulating the tran...
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Article
Open AccessFlavodiiron-mediated O2 photoreduction at photosystem I acceptor-side provides photoprotection to conifer thylakoids in early spring
Green organisms evolve oxygen (O2) via photosynthesis and consume it by respiration. Generally, net O2 consumption only becomes dominant when photosynthesis is suppressed at night. Here, we show that green thylak...
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Article
Open AccessSolar energy conversion by photosystem II: principles and structures
Photosynthetic water oxidation by Photosystem II (PSII) is a fascinating process because it sustains life on Earth and serves as a blue print for scalable synthetic catalysts required for renewable energy appl...
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Article
Open AccessStructural evidence for intermediates during O2 formation in photosystem II
In natural photosynthesis, the light-driven splitting of water into electrons, protons and molecular oxygen forms the first step of the solar-to-chemical energy conversion process. The reaction takes place in ...
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Article
Open AccessStructural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition
Light-driven oxidation of water to molecular oxygen is catalyzed by the oxygen-evolving complex (OEC) in Photosystem II (PS II). This multi-electron, multi-proton catalysis requires the transport of two water ...
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Article
Open AccessRoom temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I
Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We ...
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Article
Open AccessThomas John Wydrzynski (8 July 1947–16 March 2018)
With this Tribute, we remember and honor Thomas John (Tom) Wydrzynski. Tom was a highly innovative, independent and committed researcher, who had, early in his career, defined his life-long research goal. He w...
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Article
Open AccessUnequal misses during the flash-induced advancement of photosystem II: effects of the S state and acceptor side cycles
Photosynthetic water oxidation is catalyzed by the oxygen-evolving complex (OEC) in photosystem II (PSII). This process is energetically driven by light-induced charge separation in the reaction center of PSII...
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Article
Structures of the intermediates of Kok’s photosynthetic water oxidation clock
Inspired by the period-four oscillation in flash-induced oxygen evolution of photosystem II discovered by Joliot in 1969, Kok performed additional experiments and proposed a five-state kinetic model for photos...
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Protocol
Liquid-Phase Measurements of Photosynthetic Oxygen Evolution
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We ...
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Article
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
A robust acoustic droplet ejection–drop-on-tape method delivers samples to an X-ray free-electron laser source for combined serial femtosecond crystallography and X-ray emission spectroscopy analysis, providin...
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Article
Structure of photosystem II and substrate binding at room temperature
The structures of three intermediate states of photosystem II, which is crucial for photosynthesis, have been solved at room temperature, shedding new light on this process.
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Article
Gernot Renger (1937–2013): his life, Max-Volmer Laboratory, and photosynthesis research
Gernot Renger (October 23, 1937–January 12, 2013), one of the leading biophysicists in the field of photosynthesis research, studied and worked at the Max-Volmer-Institute (MVI) of the Technische Universität B...
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No observable conformational changes in PSII
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Article
Correction: Corrigendum: Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers
Nat. Methods 11, 545–548 (2014); published online 16 March 2014; corrected after print 3 June 2015 In the version of this article initially published, the authors claimed that with the tool cctbx.xfel, weak di...
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Chapter
Light-Dependent Production of Dioxygen in Photosynthesis
Oxygen, that supports all aerobic life, is abundant in the atmosphere because of its constant regeneration by photosynthetic water oxidation, which is catalyzed by a Mn4CaO5 cluster in photosystem II (PS II), a m...
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Article
Warwick Hillier: a tribute
Warwick Hillier (October 18, 1967–January 10, 2014) made seminal contributions to our understanding of photosynthetic water oxidation employing membrane inlet mass spectrometry and FTIR spectroscopy. This arti...
