-
Article
Open AccessAuthor Correction: Structural evidence for intermediates during O2 formation in photosystem II
-
Article
Open AccessMap** protein dynamics at high spatial resolution with temperature-jump X-ray crystallography
Understanding and controlling protein motion at atomic resolution is a hallmark challenge for structural biologists and protein engineers because conformational dynamics are essential for complex functions suc...
-
Article
Open AccessStructural evidence for intermediates during O2 formation in photosystem II
In natural photosynthesis, the light-driven splitting of water into electrons, protons and molecular oxygen forms the first step of the solar-to-chemical energy conversion process. The reaction takes place in ...
-
Article
Open AccessDe novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the ...
-
Article
Open AccessChemical crystallography by serial femtosecond X-ray diffraction
Inorganic–organic hybrid materials represent a large share of newly reported structures, owing to their simple synthetic routes and customizable properties1. This proliferation has led to a characterization bottl...
-
Article
Open AccessStructural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition
Light-driven oxidation of water to molecular oxygen is catalyzed by the oxygen-evolving complex (OEC) in Photosystem II (PS II). This multi-electron, multi-proton catalysis requires the transport of two water ...
-
Article
Open AccessRoom temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I
Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We ...
-
Article
Open AccessAn on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography
Serial femtosecond crystallography has opened up many new opportunities in structural biology. In recent years, several approaches employing light-inducible systems have emerged to enable time-resolved experim...
-
Article
Open AccessSerial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting ...
-
Article
Structures of the intermediates of Kok’s photosynthetic water oxidation clock
Inspired by the period-four oscillation in flash-induced oxygen evolution of photosystem II discovered by Joliot in 1969, Kok performed additional experiments and proposed a five-state kinetic model for photos...
-
Article
Open AccessFree-electron laser data for multiple-particle fluctuation scattering analysis
Fluctuation X-ray scattering (FXS) is an emerging experimental technique in which solution scattering data are collected using X-ray exposures below rotational diffusion times, resulting in angularly anisotrop...
-
Article
Open AccessTowards in cellulo virus crystallography
Viruses are a significant threat to both human health and the economy, and there is an urgent need for novel anti-viral drugs and vaccines. High-resolution viral structures inform our understanding of the viro...
-
Article
High-speed fixed-target serial virus crystallography
A new sample-delivery method for serial X-ray crystallography exploits the full repetition rate of the X-ray free-electron laser at the LCLS facility, thus enabling efficient, high-speed data collection to sol...
-
Article
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
A robust acoustic droplet ejection–drop-on-tape method delivers samples to an X-ray free-electron laser source for combined serial femtosecond crystallography and X-ray emission spectroscopy analysis, providin...
-
Article
Structure of photosystem II and substrate binding at room temperature
The structures of three intermediate states of photosystem II, which is crucial for photosynthesis, have been solved at room temperature, shedding new light on this process.
-
Article
De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure
BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which ...
-
Article
No observable conformational changes in PSII
-
Article
Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
A concentric-flow microfluidic electrokinetic sample injector enables efficient delivery of microcrystals in their mother liquor for serial femtosecond X-ray crystallography with minimal sample consumption.
-
Article
Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis
Synaptotagmin-1 and neuronal SNARE proteins have central roles in evoked synchronous neurotransmitter release; however, it is unknown how they cooperate to trigger synaptic vesicle fusion. Here we report atomi...
-
Article
Structure of the toxic core of α-synuclein from invisible crystals
The protein α-synuclein is the main component of Lewy bodies, the neuron-associated aggregates seen in Parkinson disease and other neurodegenerative pathologies. An 11-residue segment, which we term NACore, ap...
