24 Result(s)
within
Wolfgang P. Schröder
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Protocol
Measurements of Oxygen Evolution in Photosynthesis
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We...
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Article
Open AccessIsolation and characterization of a main porin from the outer membrane of Salinibacter ruber
Salinibacter ruber is an extremophilic bacterium able to grow in high-salts environments, such as saltern crystallizer ponds. This halophilic bacterium is red-pigmented due to the production of several carotenoid...
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Article
Open AccessHigh-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction
In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model...
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Article
Open AccessAuthor Correction: Active-site plasticity revealed in the asymmetric dimer of AnPrx6 the 1-Cys peroxiredoxin and molecular chaperone from Anabaena sp. PCC 7120
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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Protocol
Liquid-Phase Measurements of Photosynthetic Oxygen Evolution
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We ...
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Article
Open AccessActive-site plasticity revealed in the asymmetric dimer of AnPrx6 the 1-Cys peroxiredoxin and molecular chaperone from Anabaena sp. PCC 7120
Peroxiredoxins (Prxs) are vital regulators of intracellular reactive oxygen species levels in all living organisms. Their activity depends on one or two catalytically active cysteine residues, the peroxidatic ...
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Article
Open AccessIsolation of monomeric photosystem II that retains the subunit PsbS
Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was pos...
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Article
Open AccessPair-wise multicomparison and OPLS analyses of cold-acclimation phases in Siberian spruce
Analysis of metabolomics data often goes beyond the task of discovering biomarkers and can be aimed at recovering other important characteristics of observed metabolomic changes. In this paper we explore diffe...
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Article
Open AccessArabidopsisplants grown in the field and climate chambers significantly differ in leaf morphology and photosystem components
Plants exhibit phenotypic plasticity and respond to differences in environmental conditions by acclimation. We have systematically compared leaves of Arabidopsis thaliana plants grown in the field and under contr...
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Protocol
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis
For many studies regarding important chloroplast processes such as oxygenic photosynthesis, fractionation of the total chloroplast proteome is a necessary first step. Here, we describe a method for isolating t...
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Article
A novel extended family of stromal thioredoxins
Thioredoxins play key regulatory roles in chloroplasts by linking photosynthetic light reactions to a series of plastid functions. In addition to the established groups of thioredoxins, f, m, x, and y, novel p...
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Chapter and Conference Paper
Thioredoxin Interactions of the Chloroplast Lumen of Arabidopsis thaliana Indicate a Redox Regulation of the Xanthophyll Cycle
Redox signalling via thioredoxins plays central roles in the light-mediated regulation of metabolic pathways of the chloroplast. Recent observations indicate strongly that thiotransduction pathways not only ta...
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Article
The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II
The PsbP-like protein of the cyanobacterium Synechocystis sp. PCC 6803 is a peripheral component of Photosystem II, located at the lumenal side of the thylakoid membrane. Removal of this protein leads to decrease...
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Article
Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803
A recent proteomic analysis of the thylakoid lumen of Arabidopsis thaliana revealed the presence of several PsbP-like proteins, and a homologue to this gene family was detected in the genome of the cyanobacterium...
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Article
The proteome of the chloroplast lumen of higher plants
Recent research in proteomics of the higher plant chloroplast has achieved considerable progress and added to our knowledge of lumenal chloroplast proteins. This work shows that chloroplast lumen has its own s...
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Article
Update on chloroplast proteomics
Currently, relatively few proteomics studies of chloroplast have been published, but the field has just started emerging and is likely to develop more rapidly in the future. While the complex membrane structur...
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Article
Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants
The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles b...
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Article
Characterisation of an Arabidopsis thaliana cDNA encoding a novel thylakoid lumen protein imported by the ΔpH-dependent pathway
An Arabidopsis thaliana (L.) Heynh. cDNA encoding a novel 16-kDa protein (P16) of the chloroplast thylakoid lumen has been characterised. The function of the protein is unknown but it shares some sequence simila...
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Chapter
PsbX Protein from Photosystem II in Higher Plants: Localization and Gene Regulation
Photosystem H complex (PSII) of higher plants consists of more than 25 different subunits (for reviews, see ref. 1 and 2). The D1 and D2 proteins have been found to bind most, if not all of the cofactors neede...
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Chapter
PsbX Protein from Photosystem II in Higher Plants: Localization and Gene Regulation
Photosystem II complex (PSII) of higher plants consists of more than 25 different subunits (for reviews, see ref. 1 and 2). The D1 and D2 proteins have been found to bind most, if not all of the cofactors need...
