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Open AccessCorrection to: Old plasma dilution reduces human biological age: a clinical study
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Open AccessOld plasma dilution reduces human biological age: a clinical study
This work extrapolates to humans the previous animal studies on blood heterochronicity and establishes a novel direct measurement of biological age. Our results support the hypothesis that, similar to mice, h...
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Small-animal blood exchange is an emerging approach for systemic aging research
We describe a small-animal blood exchange approach developed for aging research as an alternative to heterochronic parabiosis or plasma injections. In parabiosis, animals are surgically coupled, which has seve...
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Systemic induction of senescence in young mice after single heterochronic blood exchange
Ageing is the largest risk factor for many chronic diseases. Studies of heterochronic parabiosis, substantiated by blood exchange and old plasma dilution, show that old-age-related factors are systemically pro...
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Open AccessAutologous treatment for ALS with implication for broad neuroprotection
Amyotrophic lateral sclerosis (ALS) is characterized by a progressive loss of motor neurons (MNs), leading to paralysis, respiratory failure and death within 2–5 years of diagnosis. The exact mechanisms of spo...
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Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
Simple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the ...
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Article
Open AccessK-means quantization for a web-based open-source flow cytometry analysis platform
Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called “events”) in a pop...
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Open AccessPlasma dilution improves cognition and attenuates neuroinflammation in old mice
Our recent study has established that young blood factors are not causal, nor necessary, for the systemic rejuvenation of mammalian tissues. Instead, a procedure referred to as neutral blood exchange (NBE) tha...
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Open AccessAuthor Correction: Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessSkeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellul...
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Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
Most methods for the detection of nucleic acids require many reagents and expensive and bulky instrumentation. Here, we report the development and testing of a graphene-based field-effect transistor that uses ...
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Open AccessAddendum: Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis
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Chapter
Key Age-Imposed Signaling Changes That Are Responsible for the Decline of Stem Cell Function
This chapter analyzes recent developments in the field of signal transduction of ageing with the focus on the age-imposed changes in TGF-beta/pSmad, Notch, Wnt/beta-catenin, and Jak/Stat networks. Specifically...
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Open AccessApplication of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis
Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches hav...
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Open AccessA single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
Heterochronic parabiosis rejuvenates the performance of old tissue stem cells at some expense to the young, but whether this is through shared circulation or shared organs is unclear. Here we show that heteroc...
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Correction: Corrigendum: Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells
Nature 454, 528–532 (2008); doi:10.1038/nature07034 In Fig. 1a of this Letter, the immunofluorescence images for myostatin and follistatin are inaccurate owing to many versions of this figure in multiple revis...
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Oxytocin is an age-specific circulating hormone that is necessary for muscle maintenance and regeneration
The regenerative capacity of skeletal muscle declines with age. Previous studies suggest that this process can be reversed by exposure to young circulation; however, systemic age-specific factors responsible f...
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Pharmacological inhibition of myostatin/TGF-β receptor/pSmad3 signaling rescues muscle regenerative responses in mouse model of type 1 diabetes
To study the influence of acute experimental diabetes on the regenerative potential of muscle stem (satellite) cells in mice.
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Protocol
Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells
In our studies of muscle regeneration we have developed, modified, and optimized techniques to isolate and study the stem and precursor cells to muscle tissue. Our goals have been to obtain for study muscle fi...
