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Article
Open AccessThe novel norcantharidin derivative DCZ5417 suppresses multiple myeloma progression by targeting the TRIP13–MAPK–YWHAE signaling pathway
Multiple myeloma (MM), an incurable disease owing to drug resistance, requires safe and effective therapies. Norcantharidin (NCTD), an active ingredient in traditional Chinese medicines, possesses activity aga...
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Article
Aberrant activation of TRIP13-EZH2 signaling axis promotes stemness and therapy resistance in multiple myeloma
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Article
Open AccessSirtuin 3 regulation: a target to alleviate β-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells
During the transition period, the insufficient dry matter intake and a sharply increased in energy consumption to produce large quantities of milk, high yielding cows would enter a negative energy balance (NEB...
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Article
Open AccessTargeting hyaluronic acid synthase-3 (HAS3) for the treatment of advanced renal cell carcinoma
Hyaluronic acid (HA) promotes cancer metastasis; however, the currently approved treatments do not target HA. Metastatic renal carcinoma (mRCC) is an incurable disease. Sorafenib (SF) is a modestly effective a...
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Article
Deficiency of Murine UFM1-Specific E3 Ligase Causes Microcephaly and Inflammation
The UFM1 conjugation system is a Ubiquitin (Ub)-like modification system that is essential for animal development and normal physiology of multiple tissues and organs. It consists of UFM1, a Ub-like modifier, ...
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Article
Open AccessMitofusins: from mitochondria to fertility
Germ cell formation and embryonic development require ATP synthesized by mitochondria. The dynamic system of the mitochondria, and in particular, the fusion of mitochondria, are essential for the generation of...
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Protocol
In Vitro Stimulation of IRE1α/XBP1-Deficient B Cells with LPS
During immune responses, pathogen-specific B cells differentiate into plasma cells. Plasma cells synthesize and secrete large amounts of immunoglobulin (Ig) molecules which play a central role in immunity agai...
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Article
Open AccessLong noncoding RNAs: new insights in modulating mammalian spermatogenesis
Spermatogenesis is a complex differentiating developmental process in which undifferentiated spermatogonial germ cells differentiate into spermatocytes, spermatids, and finally, to mature spermatozoa. This mul...
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Article
Open AccessEvaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells
Heat stress is known to affect follicular dynamics, oocyte maturation, and fertilization by impairing steroidogenic ability and viability of bovine granulosa cell (bGCs). The present study explored the physiol...
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Protocol
Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells
Innate lymphoid cells (ILCs) are a recently identified family of immune cells mostly present at barrier surfaces. They play an important role in the induction, regulation, and resolution of inflammatory respon...
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Article
No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas
Dosage compensation, which is achieved by X-chromosome inactivation (XCI) in female mammals, ensures balanced X-linked gene expression levels between the sexes. Although eutherian mammals commonly display rand...
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Article
Open AccessUfbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR
The IRE1α/XBP1 branch of unfolded protein response (UPR) pathway has a critical function in endoplasmic reticulum (ER) expansion in plasma cells via unknown mechanisms; interestingly, another UPR branch, PERK,...
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Article
Open AccessIntegrated analysis of mRNAs and long noncoding RNAs in the semen from Holstein bulls with high and low sperm motility
Sperm motility is the main index used to assess the quality of bull semen. It may also be used to evaluate the fertility potential of bulls. Protein-coding mRNA and long noncoding RNA (lncRNA) participate in t...
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Article
Open AccessIndispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation
Intestinal exocrine secretory cells, including Paneth and goblet cells, have a pivotal role in intestinal barrier function and mucosal immunity. Dysfunction of these cells may lead to the pathogenesis of human...
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Article
DNA methylation rather than single nucleotide polymorphisms regulates the production of an aberrant splice variant of IL6R in mastitic cows
Interleukin-6 receptor-alpha (IL6R) interacts with IL6 and forms a ligand–receptor complex, which can stimulate various cellular responses, such as cell proliferation, cell differentiation, and activation of i...
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Article
Developments in techniques for the isolation, enrichment, main culture conditions and identification of spermatogonial stem cells
The in vitro culture system of spermatogonial stem cells (SSCs) provides a basis for studies on spermatogenesis, and also contributes to the development of new methods for the preservation of livestock and ani...
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Article
Clone of Chinese **an redcross yellow cattle and evaluation of reproductive characteristics of cloned calf
Somatic cell clone technology is a viable approach to preserving endangered livestock and wildlife genetic resources. In the present research, somatic cell nuclear transfer (SCNT) was performed using granulose...
