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Article
Ultrasound Prototype for Improving Germination and Seedling Growth in Tomato and Maize Seeds
The optimization of germination rates and seedling performance is of utmost importance in the agricultural practices. Ultrasound treatment have emerged as a promising technique for seed treatment due to their ...
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Article
Open AccessGrapevine woody tissues accumulate stilbenoids following bud burst
After bud burst, a transcriptional reprogramming of the shikimate and phenylpropanoid pathways occurs in grapevine canes resulting in the accumulation of stilbenoids like resveratrol and viniferin.
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Article
Open AccessRole in Phenylpropanoid Biosynthesis of the Grapevine Plastidic Phosphoenolpyruvate Translocator VviPPT1
Phosphoenolpyruvate (PEP), the main precursor of the shikimate pathway in the plastids, is imported from the cytosol through a PEP translocator (PPT). The final product of the shikimate pathway, chorismate, is...
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Article
Early Activation of Antioxidant Responses in Ni-Stressed Tomato Cultivars Determines Their Resilience Under Co-exposure to Drought
Throughout their life cycle, plants are subjected to a variety of environmental constraints, including abiotic stresses. The present study aimed at characterizing the responses of the two tomato cultivars Gold...
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Article
The grapevine CAX-interacting protein VvCXIP4 is exported from the nucleus to activate the tonoplast Ca2+/H+ exchanger VvCAX3
The nuclear-localized CAX-interacting protein VvCXIP4 is exported to the cytosol after a Ca2+ pulse, to activate the tonoplast-localized Ca2+/H+ exchanger VvCAX3.
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Article
Open AccessCorrection to: A molecular perspective on starch metabolism in woody tissues
The original article was corrected.
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Article
Open AccessA molecular perspective on starch metabolism in woody tissues
The elucidation of the molecular mechanisms of starch synthesis and mobilization in perennial woody tissues is of the utmost scientific and agricultural importance.
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Protocol
Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells
A series of optimized protocols to isolate vacuoles from both yeast and plant cells, and to characterize the purified organelles at a functional and structural level, are described. For this purpose, we took a...
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Protocol
Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus
The isolation of vacuoles is an essential step to unravel the important and complex functions of this organelle in plant physiology. Here, we describe a method for the isolation of vacuoles from Catharanthus rose...
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Article
The grapevine VvCAX3 is a cation/H+ exchanger involved in vacuolar Ca2+ homeostasis
The grapevine VvCAX3 mediates calcium transport in the vacuole and is mostly expressed in green grape berries and upregulated by Ca 2+ ...
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Article
Low source–sink ratio reduces reserve starch in grapevine woody canes and modulates sugar transport and metabolism at transcriptional and enzyme activity levels
Severe leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter ...
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Protocol
Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
The characterization of membrane transport of specialized metabolites is essential to understand their metabolic fluxes and to implement metabolic engineering strategies towards the production of increased lev...
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Article
Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids
Two grapevine glucose-6-Pi plastidial transporters differently expressed in plant organs and in response to environmental and hormonal signals are characterized. They are involved in starch acc...
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Article
Copper homeostasis in grapevine: functional characterization of the Vitis vinifera copper transporter 1
The Vitis vinifera copper transporter 1 is capable of self-interaction and mediates intracellular copper transport.
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Article
Role of Tonoplast Proton Pumps and Na+/H+ Antiport System in Salt Tolerance of Populus euphratica Oliv.
Populus euphratica has been used as a plant model to study resistance against salt and osmotic stresses, with recent studies having characterized the tonoplast and the plasma membrane ATPases, and two Na+/H+ anti...
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Article
Open AccessPurification and functional characterization of protoplasts and intact vacuoles from grape cells
During grape berry ripening, the vacuoles accumulate water, sugars and secondary metabolites, causing great impact in plant productivity and wine quality. However, the molecular basis of these compartmentation...
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Chapter
Isolation and Use of Protoplasts from Grapevine Tissues
Highly pure, intact and functional protoplasts can be obtained from plant tissues, which are readily amenable for challenging with exogenous sugars, acids, analogues, transport inhibitors and drugs. Thus, they...
