-
Article
The evaluation of catalytic activity, reaction mechanism and catalyst classification in diesel particulate filter: a review
Diesel engines are widely used because of their high power, and diesel particulate filters can effectively control the emission of particulate matter from diesel engines, in which catalysts have a non-negligib...
-
Article
Enhanced Production of Poly-γ-glutamic Acid by Bacillus subtilis Using Stage-controlled Fermentation and Viscosity Reduction Strategy
In this study, the production of poly-γ-glutamic acid (PGA) by Bacillus subtilis using stage-controlled fermentation and viscosity reduction strategy was investigated in detail. Based on the single-factor optimiz...
-
Article
Efficient Production of Melanin by Aureobasidium Melanogenum Using a Simplified Medium and pH-Controlled Fermentation Strategy with the Cell Morphology Analysis
Natural melanin is a biopolymer with wide application prospects in medicine, food, cosmetics, environmental protection, agriculture, and so on. Microbial fermentation is an important and effective way to produ...
-
Article
Overview of mechanisms of Fe-based catalysts for the selective catalytic reduction of NOx with NH3 at low temperature
With the increasingly stringent control of NOx emissions, NH3-SCR, one of the most effective de-NOx technologies for removing NOx, has been widely employed to eliminate NOx from automobile exhaust and industrial ...
-
Article
Effects of cell physiological structure on the fermentation broth viscosity during poly-γ-glutamic acid production by Bacillus subtilis GXA-28
The high viscosity of fermentation broth limited the further improvement of PGA titer. Our previous studies indicated that adding KCl to the medium could decrease the fermentation broth viscosity and improve t...
-
Article
Open AccessAn effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
As a main drug for diseased thrombus, some clinically used thrombolytic agents have various disadvantages, safer novel thrombolytic agents are of great demand. This study aimed to achieve high and efficient pr...
-
Article
Development of a strategy for the screening of α-glucosidase-producing microorganisms
α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-d-methylglucoside (AMG) in glass plates was tested for the primary scre...
-
Article
Open AccessCost-effective fibrinolytic enzyme production by Bacillus subtilis WR350 using medium supplemented with corn steep powder and sucrose
The goal of this study was to develop a cheap and simple medium and to optimize fermentation parameters for fibrinolytic enzyme production by Bacillus subtilis WR350. A low-cost medium containing 35 g/L sucrose, ...
-
Article
Analysis of the L-malate biosynthesis pathway involved in poly(β-L-malic acid) production in Aureobasidium melanogenum GXZ-6 by addition of metabolic intermediates and inhibitors
Poly(β-L-malic acid) (PMA) is a promising polyester formed from L-malate in microbial cells. Malate biosynthesis is crucial for PMA production. Previous studies have shown that the non-oxidative pathway or oxi...
-
Article
Efficient Production of Polymalic Acid by a Novel Isolated Aureobasidium pullulans Using Metabolic Intermediates and Inhibitors
Polymalic acid (PMA) is a linear anionic polyester composed of l-malic acid monomers, which have potential applications as drug carriers, surgical suture, and biodegradable plastics. In this study, a novel strain...
-
Article
Continuous Production of Isomalto-oligosaccharides by Thermo-inactivated Cells of Aspergillus niger J2 with Coarse Perlite as an Immobilizing Material
The coarse perlite 40–80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixin...
-
Article
Open AccessProduction of poly-γ-glutamic acid by a thermotolerant glutamate-independent strain and comparative analysis of the glutamate dependent difference
Poly-γ-glutamic acid (γ-PGA) is a promising microbial polymer with wide applications in industry, agriculture and medicine. In this study, a novel glutamate-independent γ-PGA producing strain with thermotolera...
-
Article
Identification and characterization of a new agar-degrading strain with the novel properties of saccharides inhibition and nitrogen fixation
In this study, a new agar-degrading strain was isolated from soil with agar as a sole carbon source and energy. Based on its morphological, physiological, biochemical characterization and 16S rDNA sequence, th...
-
Article
A Novel Phenylalanine Ammonia-Lyase Purified from Rhodosporidium paludigenum PT3
-
Article
Purification and Characterization of a Thermostable Hypothetical Xylanase from Aspergillus oryzae HML366
In the current study, fermentation broth of Aspergillus oryzae HML366 in sugar cane bagasse was subjected to ultrafiltration and ion exchange chromatography, and two xylanases, XynH1 and XynH2, were purified. Tim...
-
Article
Genome Shuffling of Aspergillus niger for Improving Transglycosylation Activity
Isomaltooligosaccharides (IMO), the glucosylsaccharides used as food additives, are made from saccharified starch by enzymes or microbial cells with transglycosylation activity. This study aimed to generate sh...
-
Article
An integrated high-throughput strategy for rapid screening of poly(γ-glutamic acid)-producing bacteria
Poly(γ-glutamic acid) (γ-PGA) is a promising biomaterial with a wide range of unique applications. To extensively screen γ-PGA-producing bacteria with high yield and different molecular weight, we developed an...
-
Article
Two-Step Purification of a Novel β-Glucosidase with High Transglycosylation Activity and Another Hypothetical β-Glucosidase in Aspergillus oryzae HML366 and Enzymatic Characterization
Two novel β-glucosidases (BGHG1 and BGHG2) were purified from the enzyme extract of Aspergillus oryzae HML366 through nondenaturing gel electrophoresis and anion-exchange chromatography. The molecular weights for...
-
Article
Screening and Identification of a Fungal β-Glucosidase and the Enzymatic Synthesis of Gentiooligosaccharide
After screening with 0.1% esculoside and 0.03% FeCl3, we identified from rotten wood a fungal isolate HML0366 that produces high amount of β-glucosidase. Phenotypic and rDNA internal transcribed spacer sequence a...
-
Article
Induction of cellulase gene transcription by a novel oligosaccharide: molasses alcohol stillage substance
A novel cellulase inducer named MASS was isolated from molasses alcohol stillage (MAS). HPLC analysis identified it as a novel hetero-oligosaccharide composed of the monosaccharides glucose, fructose and xylos...
