9 Result(s)
within
George Iliakis
Life Sciences
English
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Article
Open AccessSCFSKP2 regulates APC/CCDH1-mediated degradation of CTIP to adjust DNA-end resection in G2-phase
The cell cycle-dependent engagement of DNA-end resection at DSBs is regulated by phosphorylation of CTIP by CDKs, the central regulators of cell cycle transitions. Cell cycle transitions are also intimately re...
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Protocol
Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage
Sucrose density gradient ultracentrifugation is a powerful technique for fractionating macromolecules like DNA, RNA, and proteins. For this purpose, a sample containing a mixture of different size macromolecul...
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Chapter
Backup Pathways of Nonhomologous End Joining May Have a Dominant Role in the Formation of Chromosome Aberrations
Chromosome aberrations are large-scale chromatin rearrangements produced in cells after exposure to ionizing radiation and other DNA-damaging agents. They have been directly implicated in cell killing, mutatio...
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Protocol
Plasmid-Based Assays for DNA End-Joining In Vitro
Double-strand breaks (DSBs) disrupt DNA integrity and cause genomic instability and cancer, mutations, or cell death. Among the pathways utilized by cells of higher eukaryotes to repair this lesion, nonhomolog...
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Protocol
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
Recent genetic and biochemical studies have provided important insights into the mechanism of nonhomologous end-joining (NHEJ) in higher eukaryotes, and have helped to characterize several components including...
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Protocol
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
The mechanisms of inhibition of DNA replication after DNA damage through the activation of the S-phase checkpoint have been the focus of several investigations over the last 40 yr. Recent studies have identifi...
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Protocol
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
A large number of studies suggests that double-strand breaks (DSBs) induced in DNA by ionizing radiation or chemical agents are critical lesions, which if unrepaired or misrepaired may kill a cell, or cause it...
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Protocol
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
Inhibition of DNA replication in eukaryotic cells was one of the earliest effects of radiation to be reported and quantitated. The elucidation of the mechanism of this inhibition has been the focus of research...
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Chapter and Conference Paper
Measurement of DNA Double Strand Breaks in Mammalian Cells: Comparison Between Pulsed Field Gel Electrophoresis and Non-Unwinding Filter Elution
DNA damage induction dose response curves were measured in synchronous and plateauphase CHO cells following exposure to sparsely ionizing radiation using pulsed-field gel electrophoresis and non-unwinding filt...
