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Protocol
G1 Premature Chromosome Condensation (PCC) Assay
Premature chromosome condensation (PCC) is a sensitive and unique way to detect interphase chromosome damage and its recovery in mammalian cells irradiated with ionizing radiation. In this chapter, we describe...
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Article
Open AccessA comprehensive analysis of radiosensitization targets; functional inhibition of DNA methyltransferase 3B radiosensitizes by disrupting DNA damage regulation
A comprehensive genome-wide screen of radiosensitization targets in HeLa cells was performed using a shRNA-library/functional cluster analysis and DNMT3B was identified as a candidate target. DNMT3B RNAi increase...
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Article
Open AccessVE-821, an ATR inhibitor, causes radiosensitization in human tumor cells irradiated with high LET radiation
High linear energy transfer (LET) radiation such as carbon ion particles is successfully used for treatment of solid tumors. The reason why high LET radiation accomplishes greater tumor-killing than X-rays is ...
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Chapter
Radiation-Induced Delayed Genome Instability and Hypermutation in Mammalian Cells
Genome instability is a hallmark of cancer cells. Inherited cancer predisposition syndromes typically show defects in DNA repair or DNA damage checkpoint systems, collectively called the DNA damage response (D...
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Article
Open AccessIn vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs
Chordoma, a rare cancer, is usually treated with surgery and/or radiation. However, very limited characterizations of chordoma cells are available due to a minimal availability (only two lines validated by now...
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Article
Open AccessDNA double-strand break induction in Ku80-deficient CHO cells following Boron Neutron Capture Reaction
Boron neutron capture reaction (BNCR) is based on irradiation of tumors after accumulation of boron compound. 10B captures neutrons and produces an alpha (4He) particle and a recoiled lithium nucleus (7Li). These...
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Chapter and Conference Paper
Measurement of DNA Double Strand Breaks in Mammalian Cells: Comparison Between Pulsed Field Gel Electrophoresis and Non-Unwinding Filter Elution
DNA damage induction dose response curves were measured in synchronous and plateauphase CHO cells following exposure to sparsely ionizing radiation using pulsed-field gel electrophoresis and non-unwinding filt...
