-
Protocol
Preventing VEGF-Mediated Vascular Permeability by Experimentally Potentiating BBB Characteristics in Endothelial Cells
Difficulties with poor reproducibility and translatability of animal model-based research, along with increased efforts to abide by the 3Rs tenet of animal welfare, are driving demand for more relevant human c...
-
Article
Open AccessIdentification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance
Endothelial cells (ECs) display remarkable plasticity during development before becoming quiescent and functionally mature. EC maturation is directed by several known transcription factors (TFs), but the speci...
-
Protocol
Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells
The use of human pluripotent stem cells (hPSCs) for modeling human diseases and therapeutic applications requires differentiation methods that generate physiologically relevant cell types in a robust and stand...
-
Article
Open AccessAlternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels
Thrombospondin-2 (TSP2) is an anti-angiogenic matricellular protein that inhibits tumor growth and angiogenesis. Tumor-associated blood vascular endothelial cells (BECs) were isolated from human invasive bladd...
-
Chapter
Requirements for Using iPSC-Based Cell Models for Assay Development in Drug Discovery
A prevalent challenge in drug discovery is the translation of findings from preclinical research into clinical success. Currently, more physiological in vitro systems are being developed to overcome some of th...
-
Article
An integrated expression atlas of miRNAs and their promoters in human and mouse
An atlas of microRNA expression patterns and regulators is produced by deep sequencing of short RNAs in human and mouse cells.
-
Article
Open AccessCharacterization of macrophage - cancer cell crosstalk in estrogen receptor positive and triple-negative breast cancer
Tumor heterogeneity may broadly influence the activation of tumor-associated macrophages. We aimed to dissect how breast cancer cells of different molecular characteristics contribute to macrophage phenotype a...
