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Article
Biosynthesis of Methoxymalonyl-acyl Carrier Protein (ACP) as an Extender Unit for Bafilomycin Polyketide in Streptomyces griseus DSM 2608
Bafilomycins belong to the 16-membered macrolactone family plecomacrolide antibiotics. In the bafilomycin biosynthetic gene cluster of Streptomyces griseus DSM 2608, five polyketide synthase (PKS) genes coding 12...
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Article
Biosynthesis of 2-amino-3-hydroxycyclopent-2-enone moiety of bafilomycin in Kitasatospora cheerisanensis KCTC2395
Bafilomycins produced by Kitasatospora cheerisanensis KCTC- 2395 belong to the 16-membered macrolactone family plecomacrolide antibiotics. Bafilomycin B1 contains 2-amino- 3-hydroxycyclopent-2-enone (C5N), a five...
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Article
Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics
Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains...
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Article
Open AccessOrganization and characterization of a biosynthetic gene cluster for bafilomycin from Streptomyces griseus DSM 2608
Streptomyces griseus DSM 2608 produces bafilomycin, an antifungal plecomacrolide antibiotic. We cloned and sequenced an 87.4-kb region, including a polyketide synthase (PKS) region, methoxymalonate genes, flaven...
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Article
Wound healing evaluation of benzalkonium chloride-loaded hydrocolloid in the wound infection model
An adequate environment for wound healing improves cell proliferation and cell activity, rearranges fibroblasts, and suppresses the multiplication of bacteria. The aim of this study was to evaluate the wound-h...
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Article
Current status and perspectives of biopharmaceutical drugs
Since the first approval of recombinant human insulin three decades ago, more than 150 biopharmaceutical drugs have been marketed, and some of them became blockbuster drugs in market size. The patent expiratio...
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Article
Biochemical characteristization of propionyl-coenzyme a carboxylase complex of Streptomyces toxytricini
Acyl-CoA carboxylases (ACC) are involved in important primary or secondary metabolic pathways such as fatty acid and/or polyketides synthesis. In the 62 kb fragment of pccB gene locus of Streptomyces toxytricini ...
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Article
Therapeutic and cost effectiveness of proton pump inhibitor regimens for idiopathic or drug-induced peptic ulcer complication
Peptic ulcer (PU) disease has a high rate of occurrence and recurrence in Korean and the selection of drug for treatment is diverse. In this study, the therapeutical effectiveness of regimens including proton ...
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Article
Open AccessThe role of acyl-coenzyme A carboxylase complex in lipstatin biosynthesis of Streptomyces toxytricini
Streptomyces toxytricini produces lipstatin, a specific inhibitor of pancreatic lipase, which is derived from two fatty acid moieties with eight and 14 carbon atoms. The pccB gene locus in 10.6 kb fragment of S. ...
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Article
Identification and characterization of acetyl-CoA carboxylase gene cluster in Streptomyces toxytricini
The gene locus for acetyl-CoA carboxylase (ACC) involved in the primary metabolism was identified from the genomic library of Streptomyces toxytricini which produces a lipase inhibitor lipstatin. The 7.4 kb clone...
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Article
Molecular cloning of the phospholipase D gene from Streptomyces sp. YU100 and its expression in Escherichia coli
The gene for phospholipase D (PLD) of Streptomyces sp. YU100 was cloned from λ phage library and hetero-logously expressed in Escherichia coli. Using an amplified gene fragment based on the consensus sequences of...
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Article
Immobilization of Streptomyces phospholipase D on a Dowex macroporous resin
The immobilization of phospholipase D produced by Streptomyces sp. YU100 was evaluated to see it would be practical for industrial applications. To accomplish this, the purified enzyme, which contained 53 unit/mg...
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Article
Production and physicochemical characterization of β-glucan produced byPaenibacillus polymyxa JB115
This study was conducted to develop a bacterial glucan as an animal feed additive. A novel glucan-producing bacterium.Paenibacillus polymyxa JB115, was isolated from Korean soil. The glucan, JB115-BG, produced by
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Article
Lactic acid fermentation of Chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model
To develop a health-aid preparation of Chinese yam (Dioscorea batatas Decne), lactic acid fermentation was attempted using a mixed starter comprising ofLactobacillus acidophilus, Streptococcus thermophilus, andBi...
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Article
Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model
In this study, a 40% ethanol extract of Chinese yam flour (Dioscoreae rhizoma), containing 177±58 μg/mL of dioscin, was tested in order to evaluate its pharmacological effects on the gastrointestinal tracts of Sp...
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Article
Cloning and sequencing of a novel glutaryl acylase β-subunit gene ofPseudomonas cepacia BY21 from bioinformatics
Pseudomonas cepacia BY21 was found to produce glutaryl acylase that is capable of deacylating glutaryl-7-aminocephalosporanic acid (glutaryl-7-ACA) to 7-aminocephalosporanic acid (7-ACA),...
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Article
Cloning, Expression, and Biological Function of a dTDP-Deoxyglucose Epimerase (gerF) Gene from Streptomyces sp. GERI-155
GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against Gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces s...
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Article
Allergenicity test of genetically modified soybean in Sprague Dawley rats
Allergenicity of genetically-modified (GM) soybean was evaluated in male Sprague Dawley rats. To confirm the CM soybean used in this study, the polymerase chain reaction (PCR) was performed using the chromosom...
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Article
Production and purification of single chain human insulin precursors with various fusion peptides
For the production and purification of a single chain human insulin precursor four types of fusion peptides β-galactosidase (LacZ), maltose binding protein (MBP), glutathione-S-transferase (GST), and (His)6-tagge...
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Article
Utilization of DNA marker-assisted selection in Korean native animals
The recent progress of DNA technologies including DNA fingerprinting (DFP) and random amplified DNA polymorphism (RAPD) analysis make it possible to identify the specific genetic traits of animals and to analy...
