-
Article
Open AccessVangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling
Wnt signaling regulates cell proliferation and cell differentiation as well as migration and polarity during development. However, it is still unclear how the Wnt ligand distribution is precisely controlled to...
-
Article
Open AccessWnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation
Wnt signaling is an evolutionarily conserved developmental pathway that is frequently hyperactivated in cancer. While multiple protein-coding genes regulated by Wnt signaling are known, the functional lncRNAs ...
-
Article
Wnts and the hallmarks of cancer
Since the discovery of the first mammalian Wnt proto-oncogene in virus-induced mouse mammary tumors almost four decades ago, Wnt signaling pathway and its involvement in cancers have been extensively investiga...
-
Article
Open AccessA Ras-LSD1 axis activates PI3K signaling through PIK3IP1 suppression
PI3K Interacting Protein 1 (PIK3IP1) is a suppressor of the PI3K/Akt/mTOR pathway. We previously reported that activated Ras suppresses PIK3IP1 expression to positively regulate the PI3K pathway in cancer cell...
-
Article
Open AccessPORCN inhibition synergizes with PI3K/mTOR inhibition in Wnt-addicted cancers
Pancreatic cancer (pancreatic ductal adenocarcinoma, PDAC) is aggressive and lethal. Although there is an urgent need for effective therapeutics in treating pancreatic cancer, none of the targeted therapies te...
-
Article
Open AccessPathogenic mutations in neurofibromin identifies a leucine-rich domain regulating glioma cell invasiveness
Glioblastoma (GBM) is the most aggressive tumor of the brain. NF1, a tumor suppressor gene and RAS-GTPase, is one of the highly mutated genes in GBM. Dysregulated NF1 expression promotes cell invasion, proliferat...
-
Article
Open AccessBone loss from Wnt inhibition mitigated by concurrent alendronate therapy
Dysregulated Wnt signaling is associated with the pathogenesis of cancers, fibrosis, and vascular diseases. Inhibition of Wnt signaling has shown efficacy in various pre-clinical models of these disorders. One...
-
Article
Open AccessCrystal structure of a PP2A B56-BubR1 complex and its implications for PP2A substrate recruitment and localization
Protein phosphatase 2A (PP2A) accounts for the majority of total Ser/Thr phosphatase activities in most cell types and regulates many biological processes. PP2A holoenzymes contain a scaffold A subunit, a cata...
-
Chapter
The Intestinal Stem Cell Niche
The intestine is one of the most rapidly proliferating tissues in the human body and serves as an important model to understand tissue stem cell function and homeostasis. The absorptive function of the intesti...
-
Article
Unwinding the Wnt action of casein kinase 1
The casein kinase 1 (CK1) family, a major intracellular serine/threonine kinase, is implicated in multiple pathways; however, understanding its regulation has proven challenging. A recent study published in Scien...
-
Article
Post-translational modifications regulate the ticking of the circadian clock
Post-translational modifications are as, or even more, important than transcriptional regulation to finely tune circadian rhythms. Oscillations in period (PER)...
-
Article
Altered Twist1 and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities
Autosomal dominant mutations in the gene encoding the basic helix-loop-helix transcription factor Twist1 are associated with limb and craniofacial defects in humans with Saethre-Chotzen syndrome. The molecular...
-
Article
Phosphopeptide map** of proteins ectopically expressed in tissue culture cell lines
Post-translational modifications such as phosphorylation play a vital role in the regulation of protein function. In our study of the basic Helix-loop-Helix (bHLH) transcription factor HAND1, it was suspected ...
-
Protocol
Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method
Protein phosphatase 2A (PP2A) is one of the major intracellular serine/threonine protein phosphatases. PP2A in vivo is an ABC heterotrimer whose substrate specificity is regulated in large part by the variable...
-
Chapter and Conference Paper
SV40 DNA Replication with Purified Proteins: Functional Interactions Among the Initiation Proteins
Simian virus 40 (SV40), has proved to be a useful model system for the study of mammalian DNA replication (Challberg and Kelly 1989; Hurwitz et al. 1990; Kelly 1988; Stillman 1989). The SV40 genome is replicated ...
