97 Result(s)
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Chun**ao Wei
Life Sciences
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Article
Ball Tonometry: A Rapid, Nondestructive Method for Measuring Cell Turgor Pressure in Thin-Walled Plant Cells
In this article we describe a new method for the determination of turgor pressures in living plant cells. Based on the treatment of growing plant cells as thin-walled pressure vessels, we find that pressures c...
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Agrobacterium-mediated genetic transformation of a Dendrobium orchid
A protocol was developed to obtain stable transgenic orchids (Dendrobium nobile) via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs). Agrobacterium tumefaciens strains AGL1 and EHA105 were u...
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Article
Loss of stability, pH, and the anisotropic extensibility of Chara cell walls
We investigated the effects of acid conditions on the extensibility of isolated wall segments from growing Chara corallina cells, providing the first detailed multi-azimuthal description of the anisotropic elasti...
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Article
Molecular Cloning and Expression of a cDNA Encoding Lon Protease from rice (Oryza sativa)
The ATP-dependent Lon protease is a highly conserved enzyme that is present in archeae, eubacteria, and eukaryotes, and plays an important role in intracellular protein degradation. We have isolated a Lon prot...
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Open AccessComparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon
White spot syndrome (WSS) is a viral disease that affects most of the commercially important shrimps and causes serious economic losses to the shrimp farming industry worldwide. However, little information is ...
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Article
Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme
High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of...
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Article
Open AccessProLoc-GO: Utilizing informative Gene Ontology terms for sequence-based prediction of protein subcellular localization
Gene Ontology (GO) annotation, which describes the function of genes and gene products across species, has recently been used to predict protein subcellular and subnuclear localization. Existing GO-based predi...
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Article
Open AccessComputational identification of ubiquitylation sites from protein sequences
Ubiquitylation plays an important role in regulating protein functions. Recently, experimental methods were developed toward effective identification of ubiquitylation sites. To efficiently explore more undisc...
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Article
Open AccessPhylogenetic analysis, subcellular localization, and expression patterns of RPD3/HDA1 family histone deacetylases in plants
Although histone deacetylases from model organisms have been previously identified, there is no clear basis for the classification of histone deacetylases under the RPD3/HDA1 superfamily, particularly on plant...
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Article
A branched-chain aminotransferase may regulate hormone levels by affecting KNOX genes in plants
Branched-chain amino acid transaminases (BCATs) play a crucial role in the metabolic pathway of leucine, isoleucine and valine by catalyzing the last step of synthesis and/or the initial step of degradation of...
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Article
Expression of rice dwarf phytoreovirus Pns6 and the specificity analysis of its monoclonal antibodies
The genome of rice dwarf phytoreovirus (RDV) is composed of 12 double-stranded RNA segments, of which segment S6 encodes a non-structural protein Pns6 identified as the movement protein. In this report, Pns6 with...
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Article
Exogenous catechin increases antioxidant enzyme activity and promotes flooding tolerance in tomato (Solanum lycopersicum L.)
We studied the extent to which catechin applied as a soil drench modifies the effects of soil waterlogging on plant growth, the functioning of the free radical scavenging system and on oxidative stress levels....
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Article
Open AccessComparison of PCR riboty** and multilocus variable-number tandem-repeat analysis (MLVA) for improved detection of Clostridium difficile
Polymerase chain reaction (PCR) riboty** is one of the globally accepted techniques for defining epidemic clones of Clostridium difficile and tracing virulence-related strains. However, the ambiguous data gener...
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Article
Open AccessPOPISK: T-cell reactivity prediction using support vector machines and string kernels
Accurate prediction of peptide immunogenicity and characterization of relation between peptide sequences and peptide immunogenicity will be greatly helpful for vaccine designs and understanding of the immune s...
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Article
Open AccessViral suppression of RNA silencing
Gene silencing (RNA silencing) plays a fundamental role in antiviral defense in plants, fungi and invertebrates. Viruses encode proteins that suppress gene silencing to counter host defense. Viral suppressors ...
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Article
Open AccessPupDB: a database of pupylated proteins
Prokaryotic ubiquitin-like protein (Pup), the firstly identified post-translational protein modifier in prokaryotes, is an important signal for the selective degradation of proteins. Recently, large-scale prot...
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Article
QSAR classification of metabolic activation of chemicals into covalently reactive species
Metabolic activation of chemicals into covalently reactive species might lead to toxicological consequences such as tissue necrosis, carcinogenicity, teratogenicity, or immune-mediated toxicities. Early predic...
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Article
Production, purification, and characterization of the cecropin from Plutella xylostella, pxCECA1, using an intein-induced self-cleavable system in Escherichia coli
Antimicrobial peptides (AMPs) are widely expressed and play an important role in innate immune defense against infectious agents such as bacteria, viruses, fungi, and parasites. Cecropins are a family of AMPs ...
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Article
Consensus QSAR model for identifying novel H5N1 inhibitors
Due to the importance of neuraminidase in the pathogenesis of influenza virus infection, it has been regarded as the most important drug target for the treatment of influenza. Resistance to currently available...
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Article
Biocatalytic resolution of benzyl glycidyl ether and its derivates by Talaromyces flavus: effect of phenyl ring substituents on enantioselectivity
Talaromyces flavus containing a constitutive epoxide hydrolase (EH) resolved racemic benzyl glycidyl ether and nine derivatives into their (R)-enantiomers. After optimization of the fermentation ...
