27 Result(s)
within
Christopher J. Secombes
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Article
Immune parameters of plaice, Pleuronectes platessa, L. along a sewage sludge gradient in the Firth of Clyde, Scotland
Plaice were caught at five stations varying in distance from the sewage sludge dum** site at Garroch Head, in the Firth of Clyde, Scotland and a number of physical and immunological parameters monitored. Sig...
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Article
Cloning, sequencing, and analysis of expression of a second IL-1 β gene in rainbow trout (Oncorhynchus mykiss)
The full-length sequence of a second IL-1 β gene (IL-1 β 2) in rainbow trout (Oncorhynchus mykiss) has been obtained. As with the first IL-...
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Article
Immunoprophylaxis in fish by injection of mouse antibody genes
Antibodies are a crucial part of the body's specific defense against infectious diseases and have considerable potential as therapeutic and prophylactic agents in humans and animals. The development of recombi...
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Fish Cytokine Genes
Relatively few cytokine and cytokine receptor genes had been sequenced in fish prior to EST and genome studies. The number of sequences is rapidly increasing and demonstrates the complexity of the cytokine net...
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Complete sequencing and expression of three complement components, C1r, C4 and C1 inhibitor, of the classical activation pathway of the complement system in rainbow trout Oncorhynchus mykiss
Three complement components, C1r, C4 and C1 inhibitor, of the classical activation pathway have been fully sequenced and their expression investigated in rainbow trout (Oncorhynchus mykiss). Trout C1r cDNA encode...
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Article
Expression in Escherchia coli and Purification of Sea Bass (Dicentrarchus labrax) Interleukin 1β, a Possible Immunoadjuvant in Aquaculture
Interleukin 1β (IL-1β) is a pleiotropic cytokine that plays a pivotal role in regulating immune responses. Our group has recently cloned IL-1β from sea bass (Dicentrarchus labrax), one of the main Mediterranean a...
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Biological Activity of Sea Bass (Dicentrarchus labrax L.) Recombinant Interleukin-1β
Biological activities of a putative mature sea bass interleukin-1β peptide, produced as a recombinant protein (rIL-1β) in Escherichia coli, have been investigated. The rIL-1β contains a 6-...
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Article
Open AccessDirectional responses following recombinant cytokine stimulation of rainbow trout (Oncorhynchus mykiss) RTS-11 macrophage cells as revealed by transcriptome profiling
The early stages of the immune response are regulated by key cytokines including both interleukin 1β (IL-1β) and interferon-γ (IFN-γ) which stimulate panels of responsive genes via conserved signal transductio...
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Article
Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts
Multiple type I interferons (IFNs) have recently been identified in salmonids, containing two or four conserved cysteines. In this work, a novel two-cysteine containing (2C) IFN gene was identified in rainbow ...
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Article
Open AccessIdentification and characterisation of a novel immune-type receptor (NITR) gene cluster in the European sea bass, Dicentrarchus labrax, reveals recurrent gene expansion and diversification by positive selection
In the last decade, a new gene family encoding non-rearranging receptors, called novel immune-type receptors (NITRs), has been discovered in teleost fish. NITRs belong to the immunoglobulin superfamily and rep...
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Article
Open AccessStarvation alters the liver transcriptome of the innate immune response in Atlantic salmon (Salmo salar)
The immune response is an energy demanding process, which has effects in many physiological pathways in the body including protein and lipid metabolism. During an inflammatory response the liver is required to...
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Article
Open AccessGenomic organisation analysis of novel immunoglobulin-like transcripts in Atlantic salmon (Salmo salar) reveals a tightly clustered and multigene family
Several novel immunoglobulin-like transcripts (NILTs) which have previously been identified in the salmonid species rainbow trout (Oncorhynchus mykiss) contain either one or two extracellular Ig domains of the V-...
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Article
Two copies of the genes encoding the subunits of putative interleukin (IL)-4/IL-13 receptors, IL-4Rα, IL-13Rα1 and IL-13Rα2, have been identified in rainbow trout (Oncorhynchus mykiss) and have complex patterns of expression and modulation
Mammalian interleukin-4 (IL-4) and IL-13 are T helper type 2 (Th2) cytokines with pleiotropic functions in immunity. They signal through receptors containing IL-4Rα and IL-2Rγ or IL-13Rα1. In addition, a decoy...
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Article
Muscle-Specific RING Finger (MuRF) cDNAs in Atlantic Salmon (Salmo salar) and Their Role as Regulators of Muscle Protein Degradation
The selection of proteins destined for degradation by the ubiquitin–proteasome pathway is coordinated by E3 ubiquitin ligases (E3Ub). One group of E3Ubs is described as muscle-specific RING finger (MuRF) molec...
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Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats
Interleukin (IL)-6, the founding member of IL-6 family cytokines, plays non-redundant roles in hematopoiesis and acute phase responses. IL-6 signals via a specific private IL-6Rα and a common beta chain gp130....
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Open AccessTranscriptomic and physiological responses to fishmeal substitution with plant proteins in formulated feed in farmed Atlantic salmon (Salmo salar)
Aquaculture of piscivorous fish is in continual expansion resulting in a global requirement to reduce the dependence on wild caught fish for generation of fishmeal and fish oil. Plant proteins represent a suit...
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Open AccessImmune gene expression profiling of Proliferative Kidney Disease in rainbow trout Oncorhynchus mykiss reveals a dominance of anti-inflammatory, antibody and T helper cell-like activities
The myxozoan Tetracapsuloides bryosalmonae is the causative agent of Proliferative Kidney Disease (PKD) targeting primarily the kidney of infected fish where it causes a chronic lymphoid immunopathology. Although...
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Open AccessInflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar)
The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance...
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Identification of the salmonid IL-17A/F1a/b, IL-17A/F2b, IL-17A/F3 and IL-17N genes and analysis of their expression following in vitro stimulation and infection
This study identifies four new IL-17A/F isoforms in salmonids, as well as IL-17N. IL-17A/F1 and IL-17A/F2 are each represented by two paralogues, with a predicted pseudogene of IL-17N also apparent in the salm...
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Article
Open AccessDisparate developmental patterns of immune responses to bacterial and viral infections in fish
During early stages of development vertebrates rely on an immature immune system to fight pathogens, but in non mammalian species few studies have taken an in-depth analysis of the transition from reliance on ...
