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Article
Recombinant antibody fragments that detect enoyl acyl carrier protein reductase in Brassica napus
Purified Brassica napus enoyl acyl carrier protein reductase (ENR) was used to select specific antibodies from a library of antibody fragments, single-chain Fv (scFv), displayed on filamentous phage. Analysis of ...
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Article
Facile assessment of cDNA constructs for expression of functional antibodies in plants using the potato virus X vector
Antibodies have been expressed in plants to confer novel traits such as virus resistance or altered phenotype. However, not every antibody is suitable for plant expression, and successful intracellular express...
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Protocol
Localization of Viral Proteins in Plant Cells: Protein Tagging
This chapter describes techniques for in vivo imaging of fluorescent fusion proteins in living cells by confocal laser scanning microscopy (CLSM). Methods are provided for (i) producing the constructs for tran...
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Protocol
Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions
This chapter describes techniques to investigate the localisation and function of virus-encoded proteins in plants using green fluorescent protein (GFP) transiently expressed from plasmids or infectious cDNA r...
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Article
Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus
The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis reveal...
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Article
Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus
We report the establishment of a hybridoma cell line secreting the monoclonal antibody (mAb) HAV, which recognizes the coat (AV1) protein of tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus. The cell ...
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Article
Complete sequence of a cryptic virus from hemp (Cannabis sativa)
Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous part...
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Article
In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)
Recombinant antibodies expressed in plants have the potential to interrupt virus infections by blocking essential stages of the infection cycle. Here, we show that the expression of a recombinant single-chain ...
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Article
Quantification of Wheat spindle streak mosaic virus and Soil borne cereal mosaic virus in resistance testing of field samples of triticale using real-time RT-PCR
Triticale, a cross between rye and wheat, is a crop important for animal feed and the production of biogas and ethanol. Soil-borne viruses found in wheat and rye, such as Furoviruses and Bymoviruses, also infe...
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Article
First detection of wheat streak mosaic virus in Germany: molecular and biological characteristics
Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all ot...
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Article
A new sequence variant of wheat spindle streak mosaic virus in Germany
Wheat spindle streak mosaic virus is a Bymovirus transmitted by the plasmodiophorid Polymyxa graminis that infects small grains such as wheat, rye and triticale. Nucleotide sequences of WSSMV isolates from severa...
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Article
Wheat yellow mosaic virus resistance in wheat cultivar Madsen acts in roots but not in leaves
Wheat yellow mosaic virus (WYMV), a member of Bymovirus, is a plant pathogenic virus transmitted by the soil-borne plasmodiophorid Polymyxa graminis. The in planta titer of the virus must...