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Protocol
Novel Approach to Measure Transepithelial Electrical Resistance in Intestinal Cells
The technique electric cell-substrate impedance sensing (ECIS) can be used to detect and monitor the behavior of intestinal cells. The methodology presented was designed to achieve results within a short time ...
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Article
Open AccessPerformance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva
Polymerase chain reaction (PCR) has proven to be the gold-standard for SARS-CoV-2 detection in clinical settings. The most common approaches rely on nasopharyngeal specimens obtained from swabs, followed by RN...
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Protocol
Correction to: Angiogenesis: Methods and Protocols
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Protocol
Quantification of Angiogenesis in Laser Choroidal Neovascularization
Murine laser-induced laser choroidal neovascularization is a widely used and robust model of wet (exudative) age-related macular degeneration (wAMD). wAMD is one of the leading causes of blindness in the Weste...
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Protocol
Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides
Cell transfection using short interfering RNAs (siRNAs) is a widely used technique to perform loss of function studies by “knocking down” genes of interest. Oftentimes, primary cells can be difficult to transf...
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Protocol
Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching
RNA-seq is a common approach used to explore gene expression data between experimental conditions or cell types and ultimately leads to information that can shed light on the biological processes involved and ...
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Protocol
Measurement of Angiogenesis, Arteriolargenesis, and Lymphangiogenesis Phenotypes by Use of Two-Dimensional Mesenteric Angiogenesis Assay
Successful therapeutic angiogenesis requires an understanding of how the myriad interactions of growth factors released during angiogenesis combine to form a mature vascular bed. This requires a model in which...
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Article
Tension in the vasculature
Blood vessels arise from progenitor cells, grow and branch by sprouting from preexisting vessels and remodel by splitting longitudinally. A new study proposes an additional mechanism. It seems that vasculariza...
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Article
Blood vessels kept quiet
Tumours must get their oxygen fix, otherwise invasive tumour growth and spread can occur. One way of quelling oxygen-deprived tumours might be through manipulating the oxygen sensor PHD2.
