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    Protocol

    Quantification of Angiogenesis in Laser Choroidal Neovascularization

    Murine laser-induced laser choroidal neovascularization is a widely used and robust model of wet (exudative) age-related macular degeneration (wAMD). wAMD is one of the leading causes of blindness in the Weste...

    Kathryn R. Green, Nicholas Beazley-Long, Amy P. Lynch, Claire L. Allen in Angiogenesis (2022)

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    Protocol

    Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides

    Cell transfection using short interfering RNAs (siRNAs) is a widely used technique to perform loss of function studies by “knocking down” genes of interest. Oftentimes, primary cells can be difficult to transf...

    Zarah B. Tabrizi, Kathryn R. Green, Amy P. Lynch, Nada S. Ahmed in Angiogenesis (2022)