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Protocol
Quantification of Angiogenesis in Laser Choroidal Neovascularization
Murine laser-induced laser choroidal neovascularization is a widely used and robust model of wet (exudative) age-related macular degeneration (wAMD). wAMD is one of the leading causes of blindness in the Weste...
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Protocol
Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides
Cell transfection using short interfering RNAs (siRNAs) is a widely used technique to perform loss of function studies by “knocking down” genes of interest. Oftentimes, primary cells can be difficult to transf...