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266 Result(s)
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Article
Production and Characterization of Growth Hormone Releasing Factor Analogs Through Recombinant DNA and Chemical Techniques
A growth hormone releasing factor analog [Leu27,Hse44]GRF(1–44)lactone has been made by recombinant DNA techniques. The peptide was expressed from a multiple–copied GRF gene fused to lacZ. The monomeric GRF pepti...
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Recombinant Human Insulin-Like Growth Factor II Expressed in Escherichia coli
Human Insulin-like growth factor II (IGF-II) was produced in Escherichia coli as a 113 amino acid chimeric protein, LE1-Met-IGF-II consisting of 45 N-terminal amino acids from the E. coli trp leader peptide and t...
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Biotransformation of aromatic aldehydes bySaccharomyces cerevisiae: Investigation of reaction rates
The rate of production ofl-phenylacetyl carbinol bySaccharomyces cerevisiae in reaction mixtures containing benzaldehyde with sucrose or pyruvate as cosubstrate was investigated in short 1 h incubations. The effe...
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Article
Identification of some industrially importantActinoplanes species
Three actinomycetes designated A294, A385 and A448 all produced novel inhibitors of an enzyme involved in the regulation of mammalian cholesterol metabolism. The organisms were identified as members of the genusA...
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Biological Production of Semisynthetic Opiates Using Genetically Engineered Bacteria
Semisynthetic derivatives of morphine and related alkaloids are in widespread clinical use. Due to the complexity of these molecules, however, chemical transformations are difficult to achieve in high yields. ...
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Engineered Chimeric Streptavidin Tetramers as Novel Tools for Bioseparations and Drug Delivery
We report the construction of chimeric streptavidin tetramers that are composed of subunits of both wild-type (WT) streptavidin and genetically-engineered streptavidin variants designed for enhanced bioseparat...
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Human hematopoietic progenitor cell isolation based on galactose-specific cell surface binding
The ability to isolate functional populations of hematopoietic progenitor cells is important to the process of hematopoietic cell transplantation and to the understanding of hematopoietic cell biology in healt...
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The induction of taxol production in the endophytic fungus—Periconia sp from Torreya grandifolia
A Periconia sp was isolated from Torreya grandifolia (a relative of yew that does not synthesize taxol) near Huangshan National Park in the People’s Republic of China. This fungus, not previously known as a tree ...
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Article
Three-dimensional cellular development is essential for ex vivo formation of human bone
Tissue engineering of human bone is a complex process, as the functional development of bone cells requires that regulatory signals be temporally and spatially ordered. The role of three-dimensional cellular i...
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Article
Serial extraction of endosperm drillings (SEED)—A method for detecting transgenes and proteins in single viable maize kernels
We have developed a method for detecting a transgene and its protein product in maize endosperm that allows the kernel to be germinated after analysis. This technique could be highly useful for several monocot...
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Engineering of complex polyketide biosynthesis — insights from sequencing of the monensin biosynthetic gene cluster
The biosynthesis of complex reduced polyketides is catalysed in actinomycetes by large multifunctional enzymes, the modular Type I polyketide synthases (PKSs). Most of our current knowledge of such systems ste...
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Article
Heterologous expression to assay for plant lectins or receptors
Heterologous expression of genes for membrane proteins can provide a useful approach to analyze ligand binding and other cell surface characteristics. We analyzed the expression and processing of a barley lect...
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Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types1. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceut...
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Article
Open AccessComplete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris
Rhodopseudomonas palustris is among the most metabolically versatile bacteria known. It uses light, inorganic compounds, or organic compounds, for energy. It acquires carbon from many types of green plant–derived...
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Open AccessA web services choreography scenario for interoperating bioinformatics applications
Very often genome-wide data analysis requires the interoperation of multiple databases and analytic tools. A large number of genome databases and bioinformatics applications are available through the web, but ...
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Article
Open AccessGlobal nucleosome occupancy in yeast
Although eukaryotic genomes are generally thought to be entirely chromatin-associated, the activated PHO5 promoter in yeast is largely devoid of nucleosomes. We systematically evaluated nucleosome occupancy in ye...
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Kinetic resolution of ketoprofen ester catalyzed by lipase from a mutant of CBS 5791
A biotransformation process was developed for the production of (S)-ketoprofen by enantioseletive hydrolysis of racemic ketoprofen ester using the mutant Trichosporon laibacchii strain CBS 5791. A satisfactory re...
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Article
Network security and data integrity in academia: an assessment and a proposal for large-scale archiving
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Open AccessA low-cost open-source SNP genoty** platform for association map** applications
Association map** aimed at identifying DNA polymorphisms that contribute to variation in complex traits entails genoty** a large number of single-nucleotide polymorphisms (SNPs) in a very large panel of in...
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Open AccessFine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura
A primary objective of comparative genomics is to identify genomic elements of functional significance that contribute to phenotypic diversity. Complex changes in genome structure (insertions, duplications, re...