12 Result(s)
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Protocol
RNA Secondary Structure Modeling Following the IPANEMAP Workflow
The structure of RNA molecules and their complexes are crucial for understanding biology at the molecular level. Resolving these structures holds the key to understanding their manifold structure-mediated func...
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Protocol
Measuring Circadian Rhythms in Human Cells
Human cells, especially primary fibroblasts from skin punch biopsy, have emerged over the last decade as powerful, unlimited, and easily accessible resources that bridge the gap between animal models and human...
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Protocol
Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment
The current absence of markers unique to MDSC, particularly those expanded during human infection, necessitate concurrent demonstration of their suppressive capacity to ensure unequivocal identification. This ...
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Protocol
A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells
The recent launch of mass cytometry or cytometry by time of flight (CyTOF) has revolutionized flow cytometry. Similar to fluorescence flow cytometry, a key challenge for CyTOF is to analyze samples of limited ...
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Protocol
Combinatorial Evolution of DNA with RECODE
In past decades, DNA engineering protocols have led to the rapid development of synthetic biology. To engineer the natural proteins, many directed evolution methods based on molecular biology have been present...
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Protocol
DNA Assembly with the DATEL Method
Simple and reliable DNA assembly methods have become a critical technique in synthetic biology. Here, we present a protocol of the recently developed DATEL (scarless and sequence-independent DNA assembly metho...
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Protocol
Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo
Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one...
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Protocol
In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain
Zebrafish (Danio rerio) is a newly emerged vertebrate animal model with a conserved gross architecture of the brain and a rich repertoire of behaviors. Due to the optical transparency and structural simplicity of...
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Protocol
Study Protein Folding and Aggregation Using Nonnatural Amino Acid p-Cyanophenylalanine as a Sensitive Optical Probe
Incorporation of nonnatural amino acids with a variety of special side groups into protein sequences has substantially expanded the experimental means of exploring protein structures and functions. Recently, p-cy...
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Protocol
Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells
Human Embryonic stem cells (hESCs) offer an invaluable tool for revealing human biology and a potential source of functional cells/tissues for regenerative medicine. The utility of hESCs will likely be signifi...
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Protocol
pSM155 and pSM30 Vectors for miRNA and shRNA Expression
MicroRNAs (miRNAs) have key roles in diverse regulatory pathways, including control of developmental timing, cell differentiation, apoptosis, cell proliferation, and organ development. miRNAs regulate gene fun...
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Protocol
Approaches to Investigate the Role of Signaling in ER-to-Golgi Transport
There have been many indications that kinases play a role in signaling the transport of proteins through the secretory pathway. Specifically, the serine/threonine kinase Akt affects the transport of cholestero...
