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Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells

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Lentivirus Gene Engineering Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 614))

Abstract

Human Embryonic stem cells (hESCs) offer an invaluable tool for revealing human biology and a potential source of functional cells/tissues for regenerative medicine. The utility of hESCs will likely be significantly enhanced and broadened by our ability to build versatile genetically modified hESC lines. Here, we describe an efficient lentiviral vector mediated method to establish stable transgenic hESCs.

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Acknowledgments

We thank Prof. Didier Trono for supplying lentiviral vectors and Prof. Keiya Ozawa for supplying the plasmid pEB2 containing IRES-Bsr.

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Authors and Affiliations

  1. Departments of Anatomy and Neurology, School of Medicine and Public Health, WiCell Institute, University of Wisconsin, Madison, WI, USA

    Zhong-Wei Du & Su-Chun Zhang

Authors
  1. Zhong-Wei Du
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  2. Su-Chun Zhang
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Corresponding author

Correspondence to Su-Chun Zhang .

Editor information

Editors and Affiliations

  1. Div. Patogenesi dei Retrovirus, Centro Nazionale AIDS, Viale Regina Elena 299, Roma, 00161, Italy

    Maurizio Federico

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Du, ZW., Zhang, SC. (2010). Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology, vol 614. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-533-0_8

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  • DOI: https://doi.org/10.1007/978-1-60761-533-0_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-532-3

  • Online ISBN: 978-1-60761-533-0

  • eBook Packages: Springer Protocols

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