6 Result(s)
within
Protocol
Dieter Swandulla
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Protocol
Identification of Subproteomic Markers for Skeletal Muscle Profiling
The biochemical and cell biological profiling of contractile fiber types and subcellular structures plays a central role in basic and applied myology. Mass spectrometry-based proteomics presents an ideal appro...
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Protocol
Histological and Histochemical Microscopy Used to Verify 2D-DIGE Pathoproteomics
Comparative gel electrophoretic analyses of normal versus pathological specimens can swiftly identify proteome-wide changes in the concentration of specific protein isoforms. The application of fluorescence tw...
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Protocol
Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers
Many biomedically relevant biomarkers are proteins with characteristic biochemical properties and a relatively restricted subcellular distribution. The comparative and mass spectrometry-based proteomic analysi...
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Protocol
Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue
Following large-scale protein separation by two-dimensional gel electrophoresis or liquid chromatography, mass spectrometry–based proteomics can be used for the swift identification and characterization of car...
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Protocol
Verification of Protein Changes Determined by 2D-DIGE Based Proteomics Using Immunofluorescence Microscopy
Fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) is a key biochemical method for the comparative analysis of complex protein mixtures. The technique focuses on the identification and chara...
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Protocol
Immunofluorescence Microscopy for DIGE-Based Proteomics
Alterations in the proteome of a tissue in different settings, as assessed by difference gel electrophoresis, can be verified for single proteins using immunohistochemistry. In fluorescence immunohistochemistr...
