11 Result(s)
within
Protocol
Da** Chen
English
Gene Expression
Human Genetics
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Protocol
Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay
MicroRNAs are post-transcriptional regulators that control mRNA stability and the translation efficiency of their target genes. Mature microRNAs are approximately 22-nucleotide in length. They mediate post-tra...
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Protocol
Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) Using Gold Nanoparticle-Based DNA Probe
Affinity purification by pulldown methods using target-bound gel beads provides a powerful approach for purifying endogenous protein complexes. Such methods can be improved by using nanoparticle-based probe, c...
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Protocol
Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-Dependent Gene Expression
The bromodomain is an evolutionarily conserved motif harbored by many transcription regulators and nearly all nuclear histone acetyltransferases including the transcriptional coactivator p300. The function of ...
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Protocol
Application of Intronic MicroRNA Agents in Cosmetics
MicroRNAs (miRNA) are short, single-stranded, noncoding RNAs that regulate a number of cellular processes through their capability to silence the expression of target proteins. Using miRNAs to target and reduc...
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Protocol
Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-Length Steroid Receptor Coactivator-1a with Steroid Receptors
Estrogens, acting via estrogen receptor (ER) play key roles in growth, differentiation, and gene regulation in the reproductive, central nervous, and skeletal systems. ER-mediated gene transcription contribute...
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Histone Deacetylase Inhibitor Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells
Pluripotent stem cells can be directed into myogenic differentiation by small molecular inducers, which preferentially activate muscle-specific transcription networks. Here we describe how to efficiently direc...
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Protocol
miRNA Expression Profiling Using Illumina Universal BeadChips
We have developed a highly sensitive, specific, and reproducible method for microRNA (miRNA) expression profiling, using BeadArray technology. This method incorporates an enzyme-assisted specificity step, a so...
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Protocol
Stem-Loop RT-qPCR for MicroRNA Expression Profiling
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan® miRNA assays en...
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Protocol
Blood-Based miRNA Preparation for Noninvasive Biomarker Development
This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The metho...
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Protocol
Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Applications
RNA-sequencing (RNA-Seq) is a digital display of a transcriptome using next-generation sequencing technologies and provides detailed, high-throughput view of the transcriptome. The first step in RNA-Seq is to ...
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Protocol
Integrative Systems Biology Approaches to Identify and Prioritize Disease and Drug Candidate Genes
Although a number of computational approaches have been developed to integrate data from multiple sources for the purpose of predicting or prioritizing candidate disease genes, relatively few of them focus on ...
