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Protocol
Blood-Based miRNA Preparation for Noninvasive Biomarker Development
This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The metho...
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Article
Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation
The DNA-binding factor SATB1 is known as a chromatin organizer. Schultze and colleagues show regulation of SATB1 expression by the transcription factor Foxp3 is necessary to confer suppression of effector cell...
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Article
Open AccessThe development of a comparison approach for Illumina bead chips unravels unexpected challenges applying newest generation microarrays
The MAQC project demonstrated that microarrays with comparable content show inter- and intra-platform reproducibility. However, since the content of gene databases still increases, the development of new gener...
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Article
Genexpressionsprofile in der onkologischen Diagnostik
Tumorerkrankungen stellen in Deutschland nach wie vor die zweithäufigste Tordesursache dar. Neben der Entwicklung wirksamer primärer und sekundärer Präventionsmaßnahmen sind vor allem Methoden zur Verbesserung...
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Protocol
RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis
Gene expression profiling of peripheral blood is a very attractive approach for the development of new diagnostic markers of blood-borne but also systemic diseases as well as the development of biomarkers for ...