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Article
Endocrine Disrupting Activities of the Flavonoid Nutraceuticals Luteolin and Quercetin
Dietary plant flavonoids have been proposed to contribute to cancer prevention, neuroprotection, and cardiovascular health through their anti-oxidant, anti-inflammatory, pro-apoptotic, and antiproliferative ac...
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Protocol
Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-Length Steroid Receptor Coactivator-1a with Steroid Receptors
Estrogens, acting via estrogen receptor (ER) play key roles in growth, differentiation, and gene regulation in the reproductive, central nervous, and skeletal systems. ER-mediated gene transcription contribute...
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Article
Open AccessClaudin 7 expression and localization in the normal murine mammary gland and murine mammary tumors
Claudins, membrane-associated tetraspanin proteins, are normally associated with the tight junctions of epithelial cells where they confer a variety of permeability properties to the transepithelial barrier. O...
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Protocol
Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
Steroid hormone receptors are members of the nuclear receptor family of ligand-activated transcriptional regulatory proteins. Recent work in the field of nuclear receptor action has demonstrated an association...
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Protocol
Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors
The green fluorescent protein (GFP) from the jellyfish, Aequoria victoria, converts blue light to green fluorescence when expressed in intact cells and transgenic animals, and has proven to be a powerful tool for...
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Article
Modulators of cellular protein phosphorylation alter thetrans-activation function of human progesterone receptor and the biological activity of progesterone antagonists
Addition of progesterone to breast cancer cellsin vivo increases phosphorylation of human progesterone receptor (PR), suggesting that phosphorylation has a regulatory role in producing the activated form of recep...
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Chapter
Mechanisms Controlling Steroid Receptor Binding to Specific DNA Sequences
The biological actions of steroid hormones are mediated by specific nuclear receptors to regulate an increase or decrease in transcription of specific genes and thereby exert their influence on development and...
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Chapter and Conference Paper
Studies of the Mechanism of Glucocorticoid Hormone Action
Within the past two decades, much information has been gained about the action of glucocorticoid hormones. These steroids penetrate the cell membrane (by mechanisms as yet unknown) and bind to soluble cellular...
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Chapter and Conference Paper
Studies on the Mechanism of Glucocorticoid Hormone Action
Extensive data are now available about many aspects of glucocorticoid hormone action. However, the fundamental mechanism through which these hormones act remains obscure. It is currently thought that most of t...
