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Article
Strong and sustained activation of the anticipatory unfolded protein response induces necrotic cell death
The endoplasmic reticulum stress sensor, the unfolded protein response (UPR), regulates intracellular protein homeostasis. While transient activation of the reactive UPR by unfolded protein is protective, prol...
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Article
Open AccessAntiestrogen Resistant Cell Lines Expressing Estrogen Receptor α Mutations Upregulate the Unfolded Protein Response and are Killed by BHPI
Outgrowth of metastases expressing ERα mutations Y537S and D538G is common after endocrine therapy for estrogen receptor α (ERα) positive breast cancer. The effect of replacing wild type ERα in breast cancer c...
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Protocol
Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-Length Steroid Receptor Coactivator-1a with Steroid Receptors
Estrogens, acting via estrogen receptor (ER) play key roles in growth, differentiation, and gene regulation in the reproductive, central nervous, and skeletal systems. ER-mediated gene transcription contribute...
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Chapter
Estrogen Receptor Induced DNA Bending
We have examined the ability of the full-length estrogen receptor (ER) and the purified estrogen receptor DNA binding domain to bend DNA on binding to estrogen response element (ERE). Purified ER DNA binding d...
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Chapter
Estrogen Control of Vitellogenin Gene Transcription and mRNA Stability
We have analyzed the regulatory strategies used to achieve the massive, estrogen-mediated, induction of the mRNA coding for the egg yolk precursor protein, vitellogenin, in primary liver cultures of the amphib...
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Chapter and Conference Paper
Estrogen Receptor Regulation of Vitellogenin Gene Transcription and Chromatin Structure
Despite remarkable advances in our understanding of the organization of eukaryotic genes, the mechanisms by which eukaryotic gene expression is regulated remain one of the major unresolved questions in biochem...
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Chapter
Ovalbumin mRNA and Ovalbumin DNA and the Molecular Biology of Steroid Hormone Action
Certain steroid hormones, including estrogens, progesterone, and testosterone have profound effects on the development and function of the hen oviduct (Oka and Schimke, 1969a, b; O’Malley et al., 1969; Palmiter a...
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Chapter
Abstracts of Submitted Papers
The mechanism of alcoholic hyperlipemia was studied in rats fed liquid diets with 36% of calories as ethanol or carbohydrate (controls). 3H was given intragastrically and 14C-lysine injected intravenously with or...
