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    Protocol

    RNA Interference (RNAi) Screening in Cultured Drosophila Cells

    Genetic perturbation assays have been crucial to the discovery of molecular pathways that drive diverse biological processes. RNA interference (RNAi)-mediated depletion of gene products represents a powerful m...

    Juan-Carlos A. Padilla, Ashley Chin, Dhara Patel… in Map** Genetic Interactions (2021)

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    Protocol

    Puromycin Labeling Coupled with Proximity Ligation Assays to Define Sites of mRNA Translation in Drosophila Embryos and Human Cells

    Genetic mutations, whether they occur within protein-coding or noncoding regions of the genome, can affect various aspects of gene expression by influencing the complex network of intra- and intermolecular int...

    Ashley Chin, Eric Lécuyer in Map** Genetic Interactions (2021)

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    Protocol

    Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex-FISH

    Determining the spatiotemporal expression dynamics of a gene, or the subcellular localization properties of its encoded RNA, is often a key first step toward elucidating its function. Fluorescent in situ hybri...

    Julie Bergalet, Carole Iampietro, Ashley Chin… in In Situ Hybridization Methods (2015)