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Article
Open AccessMulti-genome comparisons reveal gain-and-loss evolution of anti-Mullerian hormone receptor type 2 as a candidate master sex-determining gene in Percidae
The Percidae family comprises many fish species of major importance for aquaculture and fisheries. Based on three new chromosome-scale assemblies in Perca fluviatilis, Perca schrenkii, and Sander vitreus along wi...
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Article
Open AccessA Bos taurus sequencing methods benchmark for assembly, haploty**, and variant calling
Inspired by the production of reference data sets in the Genome in a Bottle project, we sequenced one Charolais heifer with different technologies: Illumina paired-end, Oxford Nanopore, Pacific Biosciences (Hi...
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Article
Open AccessA duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas)
Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating ...
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Article
Open AccessThe sterlet sturgeon genome sequence and the mechanisms of segmental rediploidization
Sturgeons seem to be frozen in time. The archaic characteristics of this ancient fish lineage place it in a key phylogenetic position at the base of the ~30,000 modern teleost fish species. Moreover, sturgeons...
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Protocol
Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex-FISH
Determining the spatiotemporal expression dynamics of a gene, or the subcellular localization properties of its encoded RNA, is often a key first step toward elucidating its function. Fluorescent in situ hybri...
