33 Result(s)
within
Biomedicine
**n** Cao
Protocol
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Protocol
Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
Stem cells have unlimited therapeutic potential for restoring CNS function lost secondary to trauma or degenerative disease. To date, stem cell grafts have only in a few instances partially ameliorated functio...
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Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage
Human embryonic stem cells (hESCs) have the ability to self-replicate and differentiate into cells from all three embryonic germ layers, thereby holding great promise for tissue regeneration applications. Howe...
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Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning
Phage display is a powerful tool in identifying specific antibody fragment binding to the target. Several strategies can be used to screen out a specific Fab from the phage library that binds to an antigen; it...
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Liver Plasma Membranes: An Effective Method to Analyze Membrane Proteome
Plasma membrane proteins are critical for the maintenance of biological systems and represent important targets for the treatment of disease. The hydrophobicity and low abundance of plasma membrane proteins ma...
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Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-Resolution Mass Spectrometry to Study the Dynamics of Site-Specific Histone Methylation
Histone lysine and arginine methylation involved in gene activation and silencing is dynamically regulated. However, partly limited to the research technologies previously available, the dynamics of global his...
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In Vivo cII, gpt, and Spi− Gene Mutation Assays in Transgenic Mice and Rats
Transgenic mutation assays are used to identify and characterize genotoxic hazards and for determining the mode of action for carcinogens. The three most popular transgenic mutational models are Big Blue® (rats o...
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Array-Based Immunoassays with Rolling-Circle Amplification Detection
This chapter describes methods for the use of antibody microarrays with rolling-circle amplification (RCA). The methods are divided into three sections. The first section covers antibody preparation and microa...
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Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice
The endogenous X-linked phosphatidyl inositol glycan class A gene (Pig-a) can be used as a reporter of in vivo somatic cell mutation in rats and mice. Pig-a mutant cells are deficient in specific protein surface ...
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Common Methods Used for the Discovery of Natural Anticancer Compounds
Carcinogenesis is a long-term, multifactorial, and multistep process. Dietary phytochemicals can play a significant role in cancer prevention. In this chapter, we describe common protocols to study the role of...
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A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
Cell migration is a critical determinant of cancer invasion and metastasis. Drugs targeting cancer cell migration have been hindered due to the lack of effective assays for monitoring cancer cell migration. He...
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Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies
The ability to manipulate capripoxvirus through gene knockouts and gene insertions has become an increasingly valuable research tool in elucidating the function of individual genes of capripoxvirus, as well as...
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The Next-Generation PCR-Based Quantification Method for Ambient Waters: Digital PCR
Real-time quantitative PCR (qPCR) is increasingly being used for ambient water monitoring, but development of digital polymerase chain reaction (digital PCR) has the potential to further advance the use of mol...
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Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like Gene Expression, RNA Editing, and MicroRNAs Regulation
Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) protein family is encoded by eleven genes located in human genome. APOBECs are a family of evolutionarily conserved cytidine deaminases...
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Identification of Transcriptional Regulators That Bind to Long Noncoding RNAs by RNA Pull-Down and RNA Immunoprecipitation
Long noncoding RNAs (lncRNAs) are RNA transcripts that are at least 200 nucleotides long and lack coding potential, and they have been demonstrated to be involved in a wide range of biological processes. Many ...
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MicroRNAs Reprogram Tumor Immune Response
Endogenously produced microRNAs (miRNAs) are predicted to regulate the translation of over two-thirds all human gene transcripts. Certain microRNAs regulate expression of genes that are critically involved in ...
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Evaluation of Protein–Ligand Docking by Cyscore
Protein–ligand docking is a powerful method in drug discovery. The reliability of docking can be quantified by RMSD between a docking structure and an experimentally determined one. However, most experimentall...
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Testing of General and Human-Associated Fecal Contamination in Waters
qPCR has become increasingly popular for microbial water quality testing because it is faster, more specific, and more flexible than culture-based methods. However, qPCR method limitations such as quantificati...
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Multisite Recording of Local Field Potentials in Awake, Free-Moving Mice
Oscillations of local field potentials (LFPs) are crucial in neuroscience studies since they are correlated with many brain activities related to sense, motor, learning, and cognition. Multisite recording of L...
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Sculpting the Sculptors: Methods for Studying the Fetal Cholinergic Signaling on Systems and Cellular Scales
The non-neuronal, immunological effects of the cholinergic signaling are exerted on the system’s scale of observation via the vagus nerve and on the cellular scale via α7 nicotinic acetylcholine receptor (nACh...
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Vaccinia Virus Transcriptome Analysis by RNA Sequencing
RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq...
