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Vaccinia Virus Transcriptome Analysis by RNA Sequencing

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Vaccinia Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells. We pay particular attention to vaccinia virus-specific aspects of sample preparation, sequencing, and data analyses, but our method could be modified to analyze transcriptomes of other cells or tissues infected with different poxviruses.

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Author information

Authors and Affiliations

  1. Division of Biology, Kansas State University, Manhattan, KS, USA

    Shuai Cao, Yongquan Lin & Zhilong Yang

  2. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tian**, China

    Yongquan Lin

Authors
  1. Shuai Cao
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  2. Yongquan Lin
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  3. Zhilong Yang
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Corresponding author

Correspondence to Zhilong Yang .

Editor information

Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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Cao, S., Lin, Y., Yang, Z. (2019). Vaccinia Virus Transcriptome Analysis by RNA Sequencing. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

  • eBook Packages: Springer Protocols

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