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Chapter and Conference Paper
RFLP Standardization Report for DR Alpha/KpnI
Four bands were identified by the RFLP analysis in the DR alpha/KPN system as shown in Table 1 and in Figure 1. RFLP patterns of the core cell lines are shown in Table 2.
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Chapter and Conference Paper
RFLP Standardization Report for DP Alpha/KpnI
Six bands were identified by the RFLP analysis in the DP alpha/KPN system as shown in Table 1 and in Figure 1. RFLP patterns of the core cell lines are shown in Table 2.
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Chapter and Conference Paper
DR3 (DRw17, DRw18): 2-D Gel Patterns
Six DR3 cell lines were included in the Workshop study (Table 1). Two-dimensional (2-D) gel patterns for both α chains and β chains were obtained using the panel of core monomorphic and selected polymorphic mo...
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Chapter and Conference Paper
Unusual DR-DQ Haplotypes
The linkage disequilibrium existing between HLA-DR and DQ antigens is so strong that associations that do not follow the pattern appear as exceptions. In Caucasoids, the associations are well known: DQw1 is li...
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Chapter and Conference Paper
Antigen Society #26 Report (DR3, DR7, DQw2): Part 1
There were 1,019 cells analyzed in the Antigen Society (795 Caucasians, 202 Negroes, 22 Orientals); 1,011 were tested both on Core serology and Antigen Society reagents. The total number of sera and monoclonal...
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Chapter and Conference Paper
DQw2: 2-D Gel Patterns
The pH gradient used in this study was not optimal for the two-dimensional (2-D) gel analysis of the DQβ chains from DQw2 cell lines. Therefore, only the most acidic, highly glycosylated form of this extremely...
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Chapter and Conference Paper
DQw4 (Wa): 2-D Gel Patterns
DQw4 (Wa) is a class II specificity defined in the 10th Workshop as a fourth allele at the DQ locus. It is revealed serologically with the mouse monoclonal antibody Hu-46, which reacts with some DR4 and DRw8 h...
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Chapter and Conference Paper
Antigen Society #2 Report (A25, A26, AW34, AW43 and AW66)
The HLA antigens A25, A26, AW34, AW43, and AW66 form a strongly cross-reacting group as most sera to these antigens react with two or more of these specificities. Monospecific sera against some of these specif...
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Chapter and Conference Paper
Antigen Society #12 Report (Bw54, Bw55, Bw56 and Bw42)
The antigen Bw54 was officially designated as a split of Bw22 at the Seventh International Histocompatibility Workshop. This antigen was primarily defined in the Japanese population by several laboratories ind...
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Chapter and Conference Paper
Antigen Society #18 Report (Cw4 and Cw6)
HLA-Cw4 (formerly T4, RH315) and HLA-Cw6 (formerly T7) obtained the formal HLA designation after the Sixth and Seventh International Histocompatibility Workshops respectively (1,2). Since then, it has been obs...
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Chapter and Conference Paper
Southern Blot Analysis of Rheumatoid Arthritis
The methods and materials used in the Southern blot analysis of rheumatoid arthritis (RA) study were identical to the conditions used in the core and family study sections of the Tenth Workshop (10WS) Southern...
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Chapter and Conference Paper
DRw6 (DRw13, DRw14): 2-D Gel Patterns
DRw6 (DRw13,DRw14) haplotypes were previously considered to be the most enigmatic group of HLA class II specificities. This was largely due to the lack of monospecific anti-DRw6 alloantisera. Through the combi...
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Chapter and Conference Paper
Antigen Society #25 Report DRw6, DRw13, DRw14)
Since the Ninth International Histocompatibility Workshop 1984, two splits of HLA-DRw6 have been recognized: DRw13 and DRw14(1). Both specificities were included in DRw52 and highly associated with DQw1. At th...
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Chapter and Conference Paper
DQw1 (DQw5, DQw6): 2-D Gel Patterns
The HLA-DQw1 specificity was the first supertypic specificity recognized and was described at the 7th Workshop(1). Structural differences have been demonstrated between DQ molecules associated with DR1, DR2, a...
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Chapter and Conference Paper
DRw52: 2-D Gel Patterns
The HLA-DRw52 specificity comprises a group of supertypic determinants, normally expressed on DR3, DR5, DRw6, and DRw8 positive cells, which can be detected by means of alloantisera (1), monoclonal antibodies ...
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Chapter and Conference Paper
Antigen Society #7 Report (B8, w59, B14, w64, w65)
HLA B8 and HLA B14 have been well established specificities since 1965 (1) and 1970 (2), respectively. Bw59, restricted to the Oriental population, was defined in 1980 (3), and the B14 subtypes Bw64 and Bw65 w...