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2,056 Result(s)
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Article
Two-phase mixed indicator method for the determination of zwitterionic surfactants
The two-phase, mixed indicator method for anionic surfactants has been modified and applied to zwitterionic surfactants. The modifications entail: (i) lowering the pH; (ii) adding 95% ethanol, and (iii) increa...
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Article
Cellulase production from spent sulfite liquor and paper-mill waste fiber
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Article
The principle of parabolic feed for a fed-batch culture of baker’s yeast
A parabolic nutrient supplementation has been derived by the specific growth rate, sugar conversion rate, and Pasteur effect, followed by high qualitative and quantitative biomass production with the lowest su...
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Xanthan fermentations in water/oil dispersions
Xanthan fermentations in W/O dispersions performed better than the control in both small flasks and a 6.6-L fermentor. The better bulk mixing and oxygen transfer achieved in the dispersion resulted in a still ...
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Article
The microbial production of polyhydroxybutyrate from methanol
One hundred strains of methanol-utilizing bacteria were isolated. From them a methylotrophic strain 8502-3 was selected out for biodegradable plastics polyhydroxybutyrate (PHB) production on methanol. It was n...
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Beam-induced reaction between meta-nitrobenzyl alcohol and dipyridocyanine dyes in liquid-secondary-ion mass spectrometry
Analyses of cationic dipyridocyanine dyes by liquid-secondary-ion mass spectrometry in a liquid matrix of meta-nitrobenzyl alcohol (rnNBA) provide evidence for beam-induced addition reactions between the sample m...
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Microbial transformation of 12-hydroxyoctadecanoic acid to 5-n-hexyl-tetrahydrofuran-2-acetic acid
Stationary-phase cells of a corynebacterium (FUI-2) and a bacillus (NRRL B-14864) isolate, when grown aerobically in 1% YE medium at 25°C, converted 12-hydroxystearic acid to a major compound, 5-n-hexyl-tetrahydr...
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Production and properties of 3-cyanopyridine hydratase inRhodococcus equi SHB-121
A strain ofRhodococcus equi SHB-121 forming 3-cyanopyridine hydratase was screened from nitrile-polluted soil. The optimum conditions for the formation of 3-cyanopyridine hydratase by the strain SHB-121 have been...
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Production, characterization, and application of the cellulase-free xylanase fromAspergillus niger
The effects of carbon source on xylanase and cellulase production were studied. The extract of steam-exploded corn stover was found to be the best raw material for producing cellulase-free xylanase. The xylano...
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Estimation of the optimal concentrations of residual sugar and cell growth rate for a fed-batch culture ofSaccharomyces cerevisiae
Estimation of the optimal concentrations of residual sugar in medium for a fed-batch culture of Baker’s yeast has been studied and practiced. The concentrations, however, depended on different species and targ...
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Article
Dissociation of heme-globin complexes by blackbody infrared radiative dissociation: Molecular specificity in the gas phase?
The temperature dependence of the unimolecular kinetics for dissociation of the heme group from holo-myoglobin (Mb) and holo-hemoglobin α-chain (Hb-α) was investigated with blackbody infrared radiative dissociati...
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Article
The analysis of salen complexes by fast atom bombardment mass spectrometry and atmospheric pressure chemical ionization mass spectrometry
Fast atom bombardment (FAB) mass spectrometry provides useful structural information about salen complexes and salen-based oxo transfer catalysts that are not appreciably soluble in organic solvents. It was di...
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The production and properties of a new xylose reductase from fungus
Neurospora crassa XI was found to ferment xylose and glucose simultaneously. Xylose was the appropriate inducer for the production of xylose reductase that had two isoenzymes designated as EI and...
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Article
O2 transfer to pseudoplastic fermentation broths in air-lift reactors with different inner designs
The effect of two different draft tube designs on annulus air-lift reactor (AALR) performance was studied using the pseudoplastic fluid such as sodium carboxymethyl cellulose (CMC) and Streptomyces lincolnesis...
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Effects of mixing conditions on the production of microbial cellulose byAcetobacter xylinum
Microbial cellulose has many potential applications due to its excellent physical properties. The production of cellulose fromAcetobacter xylinum in submerged culture is, however, beset with numerous problems. Th...
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Article
Oxygen transfer characteristics of multiple-phase dispersions simulating water-in-oil xanthan fermentations
A water-in-oil (W/O) cultivation technology has the potential of overcoming the problems related with high broth viscosity in xanthan fermentations. The aqueous broth is dispersed in a continuous oil phase. C...
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Article
Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction
An efficient and simple method for removing known, abundant cDNA species from the cDNA pool of highly differentiated cells is reported. The method involves preparation of sscDNA, followed by dscDNA-synthesis o...
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Article
Detection of nitric oxide in tissue by spin trap** EPR spectroscopy and triacetylglycerol extraction
A modified method based on EPR spin trap** and triacetylglycerol extraction was used for tissue nitric oxide (NO) detection at room temperature. NO signal intensity was stable for about 1.5 h and the detecti...
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Article
Molecular analysis and heterologous expression of the gene encoding methylmalonyl—coenzyme a mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32
The conversion of succinyl-coenzyme A (CoA) into methylmalonyl-CoA, catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase (MCM), represents an important source of building blocks for rifamycin SV b...
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A novel magnetite-immobilized cell process for heavy metal removal from industrial effluent
The sorption and desorption of copper (II) (Cu[II]) ions from the wastewater by magnetite-immobilized cells of Pseudomonas putida 5-x with acidic pretreatment were studied. Pretreating cells with 0.6 N HCl was fo...