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Detection of nitric oxide in tissue by spin trap** EPR spectroscopy and triacetylglycerol extraction

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Biotechnology Techniques

Abstract

A modified method based on EPR spin trap** and triacetylglycerol extraction was used for tissue nitric oxide (NO) detection at room temperature. NO signal intensity was stable for about 1.5 h and the detection limit of this method was less than 200 pmol g−1 tissue. Using this method, we report evidence that NO production in vivo can be inhibited by adriamycin in mice livers.

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Zhou, G., Zhao, B., Hou, J. et al. Detection of nitric oxide in tissue by spin trap** EPR spectroscopy and triacetylglycerol extraction. Biotechnology Techniques 13, 507–511 (1999). https://doi.org/10.1023/A:1008977314541

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  • DOI: https://doi.org/10.1023/A:1008977314541

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