Abstract
The effects of carbon source on xylanase and cellulase production were studied. The extract of steam-exploded corn stover was found to be the best raw material for producing cellulase-free xylanase. The xylanolytic enzymes ofAspergillus niger An-76 were purified by chromatography, and the properties of the four purified components were analyzed. When the enzyme was used to treat the birch Kraft pulp, and followed by a subsequent CEH bleaching, a brightness of 6.8% SBD more than that of the untreated one using the same chlorine dosage, or a saving of nearly 50% of chlorine consumption with the same brightness, was realized.
Similar content being viewed by others
References
Higuchi, T. (1989), inBioprocess Engineering, Ghose, T. K., ed., Ellis Horwood, Chichester, UK, pp. 39–58.
Viikari, L., Kantelinen, A., Poutanen, K., and Ranua, M. (1986), 3rd Int. Conference on Biotechnology in the Pulp and Paper Industry, Stockholm, June 16–19, pp. 67–69.
Viikari, L., Kantelinen, A., Siika-aho, M., Tenkanen, M., Buchert, J., Bailey, M., Pere, J., and Linko, M. (1993), inTrichoderma reesei Cellulase and Other Hydrolases, Suomium, P. and Reinikaineu, T., eds., Helsinki, Finland, pp. 255–262.
Kautelium, A., Hortling, B., Sundquist, J., Linko, M., and Viikari, L. (1993),Holzforschung 47, 318–324.
Chen, H. Z., Gao, P., and Wang, Z. (1990),Acta Microbiologica Sinica 30, 351–357.
Beldman, G., Voragen, A. G. J., Rombouts, F. M., Searle-van Leeuwen, M. F., and Pilnik, W. (1988),Biotechnol. Bioeng. 31, 160–167.
Zeng, Y. C. and Zhang, S. Z. (1993),Acta Microbiologica Sinica 33, 427–433.
Gomes, J., Sinner, M., and Steiner, W. (1993),Enzyme Microb. Technol. 15, 677–682.
Mandels, M. and Reese, E. T. (1957),J. Bacteriol. 73, 269–278.
Ghose, T. K. (1987),Pure Appl. Chem. 59, 2557–2568.
Gomes, I., Gomes, J., Steiner, W., and Esterbauer, H. (1992),Appl. Microbiol. Biotechnol. 36, 701–707.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yinbo, Q., Peiji, G., Dong, W. et al. Production, characterization, and application of the cellulase-free xylanase fromAspergillus niger . Appl Biochem Biotechnol 57, 375–381 (1996). https://doi.org/10.1007/BF02941716
Issue Date:
DOI: https://doi.org/10.1007/BF02941716