38 Result(s)
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Article
γ-Cyclodextrin: a review on enzymatic production and applications
Cyclodextrins are cyclic α-1,4-glucans that are produced from starch or starch derivates using cyclodextrin glycosyltransferase (CGTase). The most common forms are α-, β-, and γ-cyclodextrins. This mini-review...
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Article
Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis
LadA, a monooxygenase catalyzing the oxidation of n-alkanes to 1-alkanols, is the key enzyme for the degradation of long-chain alkanes (C15–C36) in Geobacillus thermodenitrificans NG80-2. In this study, random- a...
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Article
Comparative study of four rice cultivars with different levels of cadmium tolerance
Cadmium (Cd) has been identified as a significant pollutant due to its high solubility in water and soil and high toxicity to plants and animals. Rice, as one of the most important food crops, is grown in soil...
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Article
Genomic structural variations contribute to trait improvement during whole-genome shuffling of yeast
Whole-genome shuffling (WGS) is a powerful technology of improving the complex traits of many microorganisms. However, the molecular mechanisms underlying the altered phenotypes in isolates were less clarified...
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Article
Open AccessOpen conformation of hERG channel turrets revealed by a specific scorpion toxin BmKKx2
The human ether-a-go-go-related gene potassium channel (hERG) has an unusual long turret, whose role in recognizing scorpion toxins remains controversial. Here, BmKKx2, the first specific blocker of hERG chann...
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Article
Physicochemical properties and membrane biofouling of extra-cellular polysaccharide produced by a Micrococcus luteus strain
The physicochemical properties of the extra-cellular polysaccharide (EPS) produced by a Micrococcus luteus strain, a dominating strain isolated from membrane biofouling layer, were determined in t...
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Article
Colwellia arctica sp. nov., isolated from Arctic marine sediment
Strain 435T, a catalase- and oxidase-positive, beige-pigmented, facultatively anaerobic and Gram-stain-negative marine bacterium, was isolated from marine sediment collected in the Arctic (8°21′629′′E 72°8′827′′N...
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Article
Sulfide oxidation and nitrate reduction for potential mitigation of H2S in landfills
Because H2S emitted by landfill sites has seriously endangered human health, its removal is urgent. H2S removal by use of an autotrophic denitrification landfill biocover has been reported. In this process, nitra...
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Article
Sphingomonas flavus sp. nov. isolated from road soil
A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5T, was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence,...
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Article
Hydrogenophaga luteola sp. nov. isolated from reed pond water
A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7T, was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analy...
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Article
Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond
A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326T, was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122°14′34″E...
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Article
Identification of three PPV1 VP2 protein-specific B cell linear epitopes using monoclonal antibodies against baculovirus-expressed recombinant VP2 protein
Porcine parvovirus type 1 (PPV1) is a major causative agent of embryonic and fetal death in swine. The PPV1 VP2 protein is closely associated with viral immunogenicity for eliciting neutralizing antibodies, bu...
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Article
Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein
The pseudorabies virus (PRV) UL42 protein, known as the DNA polymerase processivity factor, is an essential protein required for viral replication. The in vitro function of UL42 has been characterized; however...
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Article
Characterization and application of monoclonal antibodies against Mycoplasma hyorhinis pyruvate dehydrogenase E1 complex subunit alpha
Mycoplasma hyorhinis is commonly found in the respiratory tract of pigs and is the etiological agent of polyserositis. The metabolic enzymes of M. hyorhinis may play important roles in ho...
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Article
Acinetobacter plantarum sp. nov. isolated from wheat seedlings plant
Strain THG–SQM11T, a Gram-negative, aerobic, non-motile, coccus-shaped bacterium, was isolated from wheat seedlings plant in P. R. China. Strain THG–SQM11T was closely related to members of the genus Acinetobact...
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Article
Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s
Two major porcine circovirus type 2 (PCV2) genotypes, PCV2a and PCV2b, are recognized. PCV2a was predominant in the global pig population until 2000 while PCV2b became predominant from 2003 onward. The aim of ...
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Article
Isolation and characterization of Vibrio alginolyticus from cultured amphioxus Branchiostoma belcheri tsingtauense
Amphioxus is an intermediary species bridging invertebrates and vertebrates used to study the development and evolution of species and genes. In this study, the dominant bacterial strain was isolated from tail...
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Article
Enhanced delivery of biodegradable mPEG-PLGA-PLL nanoparticles loading Cy3-labelled PDGF-BB siRNA by UTMD to rat retina
We investigated the efficacy and safety of ultrasound (US)-targeted microbubble (MB) destruction (UTMD)-enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-l-lysine (mPEG-PLGA...
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Article
Genetic diagnosis of a Chinese multiple endocrine neoplasia type 2A family through whole genome sequencing
Approximately 98% of patients with multiple endocrine neoplasia type 2A (MEN 2A) have an identifiable RET mutation. Prophylactic or early total thyroidectomy or pheochromocytoma/parathyroid removal in patients ca...
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Article
Open AccessDysregulation of NCAPG, KNL1, miR-148a-3p, miR-193b-3p, and miR-1179 may contribute to the progression of gastric cancer
Emerging evidence indicate that miRNAs play an important role on gastric cancer (GC) progression via regulating several downstream targets, but it is still partially uncovered. This study aimed to explore the ...
