Abstract
Strain THG–SQM11T, a Gram-negative, aerobic, non-motile, coccus-shaped bacterium, was isolated from wheat seedlings plant in P. R. China. Strain THG–SQM11T was closely related to members of the genus Acinetobacter and showed the highest 16S rRNA sequence similarities with Acinetobacter junii (97.9 %) and Acinetobacter kookii (96.1 %). DNA–DNA hybridization showed 41.3 ± 2.4 % DNA reassociation with A. junii KCTC 12416T. Chemotaxonomic data revealed that strain THG–SQM11T possesses ubiquinone-9 as the predominant respiratory quinone, C18:1 ω9c, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and C16:0 as the major fatty acids. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylcholine. The DNA G+C content was 41.7 mol %. These data, together with phenotypic characterization, suggest that the isolate represents a novel species, for which the name Acinetobacter plantarum sp. nov. is proposed, with THG–SQM11T as the type strain (=CCTCC AB 2015123T =KCTC 42611T).
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Acknowledgments
This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) and The High-end Foreign Expert Project of China, No. GDT20152100019.
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Communicated by Erko Stackebrandt.
The NCBI GenBank accession numbers for the 16S rRNA gene sequence and rpoB gene sequence of strain THG–SQM11T are KM598254 and KR856237, respectively.
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Supplementary Fig. S1. Maximum-likelihood tree based on 16S rRNA gene sequences showing the relationships between strain THG–SQM11T and other related type species. Numbers at nodes (over 50% are shown) represent percentages of bootstrap support based on a maximum-likelihood analysis of 1,000 resampled datasets. Moraxella lacunata ATCC 17967T (AF005160) was used as outgroup (PDF 13 kb)
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Supplementary Fig. S2. Neighbour-joining phylogenetic tree based on rpoB gene sequences showing the relationships of strain THG–SQM11T with related Acinetobacter species. Bootstrap values (expressed as percentage of 1,000 replications) over 70% are shown at branch points. Bar, 0.02 substitutions per nucleotide position (PDF 15 kb)
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Supplementary Fig. S3. Transmission electron micrograph of cells of THG–SQM11T. The detection was performed after negative staining with uranyl acetate. Bar, 0.5µm (PDF 128 kb)
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Supplementary Fig. S4. Two-dimensional thin-layer chromatography of polar lipids of strain THG–SQM11T (a1, a2, and a3) and A. junii KCTC 12416T (b1, b2, and b3). a1 and b1: Total lipids detected by spraying with 5 % molybdophosphoric acid; a2 and b2: aminolipids detected by spraying with 0.2 % ninhydrin; a3 and b3: phospholipids detected by spraying with molybdenum blue. Abbreviations: Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), unidentified aminolipid (AL), unidentified lipid (L) (PDF 120 kb)
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Du, J., Singh, H., Yu, H. et al. Acinetobacter plantarum sp. nov. isolated from wheat seedlings plant. Arch Microbiol 198, 393–398 (2016). https://doi.org/10.1007/s00203-016-1199-3
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DOI: https://doi.org/10.1007/s00203-016-1199-3