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Article
Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...
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Article
Open AccessDemonstration of targeted crossovers in hybrid maize using CRISPR technology
Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant ...
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Article
“Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants
A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.
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Article
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
We obtained transgenic maize plants by using high-velocity microprojectiles to transfer genes into embryogenic cells. Two selectable genes were used to confer resistance to either chlorsulfuron or phosphinothr...
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Article
Medical miscellany
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Article
Medical miscellany