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Article
Mineralization and Alkaline Phosphatase Activity in Collagen Lattices Populated by Human Osteoblasts
Adult human osteoblastic cells were grown in a native type I collagen gel. Proliferation and viability analyses showed that cells rapidly stopped dividing and became blocked in the G0G1 phase (91% on day 13)....
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Article
35th Annual Meeting of the European Association for the Study of Diabetes
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Article
Characterization of human osteoblastic cells: Influence of the culture conditions
Human osteoblastic cells were isolated enzymatically from adult human spongy bone and grown in MEM-Ham F12 1:1 medium supplemented with 2% Ultroser (USM). They were subcultured and examined for osteoblast feat...
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Article
Morphological alteration of fibroblasts mechanically stressed in a collagen lattice
The behavior of fibroblast-populated collagen lattices under mechanical stress was studied. Lattice retraction was blocked to allow the application of mechanical stress by contraction of the fibr...
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Chapter
Multi-level reasoning for managing distributed enterprises and their networks
In this paper we describe our experiences and directions regarding the management of multi-domain Enterprise Networks (ENs). Our views are (i) the management of the EN is a distributed problem-solving activity...
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Article
An improved scheme of leucine derivative fragmentation in mass spectrometry
This study illustrates the contribution of stable isotopes to amino acid mass spectrometry. The mass spectra of natural leucine and 113C leucine were compared and the mass fragmentography pattern analysed. This a...
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Chapter
Transcription Level of CHO Cells Influenced by Amino Acids
The transcription level of CHO-DUKX cells could be altered by targeted omitting of the nonessential amino acids asparagine and serine in the serum-free culture medium. These changes in transcription activity w...
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Chapter
Genetic Manipulations of Members of the Family Pasteurellaceae
The family Pasteurellaceae is comprised of three genera: Haemophilus, Actinobacillus, and Pasteurella (HAP). These bacteria cause important diseases in humans and animals and are of great medical and economical i...
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Chapter
Exotoxins of Actinobacillus Pleuropneumoniae
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae) is the etiological agent of swine pleuropneumonia, a severe, contagious disease of swine causing important economic losses ...
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Article
HLA class II genes and antibodies against recombinant U1-nRNP proteins in patients with systemic lupus erythematosus
To investigate a possible involvement of HLA-class II alleles in the genetic predisposition for the formation of anti-U1-nRNP antibody in systemic lupus erythematosus (SLE), genomic DNA of 178 patients was typ...
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Article
Separation of amino acids using ion-paired reversed-phase high-performance liquid chromatography with special reference to collagen hydrolysate
The collagen study includes the analysis of its characteristic amino acids: proline, hydroxyproline, lysine, hydroxylysine. HPLC offers an interesting device if associated with on-line radiometric detection fo...
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Chapter
Iterons of stringently controlled plasmids and DNA fingerprinting
DNA probes which detect polymorphic, repetitive sequences in a variety of genomes have been developed using different approaches. Naturally occurring plasmids, with repeated units termed iterons near or within...
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Chapter
Nonlinear Magnetooptics. The Photoinduced Faraday Rotation in Diluted Magnetic Semiconductors
We analyse the light mediated mechanisms that give rise to the giant photoinduced Faraday rotation recently observed in diluted magnetic semiconductors.
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Article
A direct fluorimetric DNA assay on cell suspensions
An improved direct fluorimetric assay is described using bisbenzimidazol fluorescence at 356 nm excitation and 458 nm emission wavelengths. The turbidity of the medium was shown to have no effect on fluorescen...
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Article
Study of basement membrane formation in dermal-epidermal recombinants in vitro
Two different dermal-epidermal recombinants were prepared in vitro and used to study the synthesis and formation of basement membrane. The first was obtained by culturing keratinocytes on the surface of a coll...
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Article
Collagen biosynthesis and isomorphism in a case of Ehlers-Danlos syndrome Type VI
Collagen metabolism was studied in fibroblasts grown from a skin biopsy specimen of a patient who presented the striking clinical features of Ehlers-Danlos syndrome and, in particular, hyperextensibility of th...
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Chapter and Conference Paper
Synthesis and Biological Activity of Agonists for the Neuronal Tachykinin Receptor in Guinea Pig Ileum
The classification of tachykinin receptors into two subclasses, the SP-P and the SP-E receptors has been well established (1). Recently Laufer et. al. (2) characterized, in the guinea pig ileum, a third tachyk...
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Article
Ω mutagenesis in gram-negative bacteria: A selectable DNA fragment which terminates transcription in a wide range of bacterial species
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Article
Behavior of the fibroblasts in recessive epidermolysis bullosa dystrophica within a collagen lattice
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Chapter
Mutagenesis
Bacterial plasmids are autonomously replicating mini chromosomes (replicons) which contain genetic information for many different properties, including the production of toxins and adhesion antigens, resistanc...
