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Article
40th EASD Annual Meeting of the European Association for the Study of Diabetes
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Article
In Vitro Glycoxidation of Insoluble Fibrous Type I Collagen: Solubilization and Advanced Glycation End Products
The deleterious effects of glycoxidation are dependent on the half-life of proteins. Collagen, the main component of extracellular matrices, is a long live protein and thus may be sensitive to the glycoxidatio...
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Article
Changes in Glycation of Fibrous Type I Collagen During Long-Term In Vitro Incubation with Glucose
The course of glycation of calf skin fibrous type I collagen was monitored in vitro under physiological conditions during an 8-week incubation period in order to take into account the long half-life of this prote...
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Article
Production of matrix metalloproteinase 2 in fibroblast reaction to mechanical stress in a collagen gel
The reaction of fibroblasts to mechanical forces generated by fibroblasts themselves in anchored collagen lattices was studied. Fibroblasts were cast in collagen gels. Free retracted gels (RG) were compared w...
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Article
Monitoring of ascorbate at a constant rate in cell culture: Effect on cell growth
Ascorbic acid (vitamin C) is a primary antioxidant for cells. But, ascorbic acid added to culture medium is not readily available to cells in culture, because it is unstable in aqueous media. We determined the...
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Article
Mineralization and Alkaline Phosphatase Activity in Collagen Lattices Populated by Human Osteoblasts
Adult human osteoblastic cells were grown in a native type I collagen gel. Proliferation and viability analyses showed that cells rapidly stopped dividing and became blocked in the G0G1 phase (91% on day 13)....
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Article
Urine glycyl-L-proline increase and skin trophicity
Glycyl-L-proline (gly-pro) is an end product of collagen metabolism that is further cleaved by prolidase (EC 3.4.13.9); the resulting proline molecules are recycled into collagen or other proteins. We postulat...
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Article
A comparison of15N proline and13C leucine for monitoring protein biosynthesis in the skin
The tracers L15N-proline and L(1-13C)-leucine were used to explore the synthesis of skin proteins in vivo in rabbits. They orally received a single dose containing an equimolecular mixture of L(1-13C)-leucine and...
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Article
Morphological alteration of fibroblasts mechanically stressed in a collagen lattice
The behavior of fibroblast-populated collagen lattices under mechanical stress was studied. Lattice retraction was blocked to allow the application of mechanical stress by contraction of the fibr...
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Article
Separation of amino acids using ion-paired reversed-phase high-performance liquid chromatography with special reference to collagen hydrolysate
The collagen study includes the analysis of its characteristic amino acids: proline, hydroxyproline, lysine, hydroxylysine. HPLC offers an interesting device if associated with on-line radiometric detection fo...
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Article
Study of basement membrane formation in dermal-epidermal recombinants in vitro
Two different dermal-epidermal recombinants were prepared in vitro and used to study the synthesis and formation of basement membrane. The first was obtained by culturing keratinocytes on the surface of a coll...
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Article
Collagen biosynthesis and isomorphism in a case of Ehlers-Danlos syndrome Type VI
Collagen metabolism was studied in fibroblasts grown from a skin biopsy specimen of a patient who presented the striking clinical features of Ehlers-Danlos syndrome and, in particular, hyperextensibility of th...
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Article
Behavior of the fibroblasts in recessive epidermolysis bullosa dystrophica within a collagen lattice
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Article
In-vitro responses to ascorbate and manganese in fibroblasts from a patient with prolidase deficiency and iminodipeptiduria: Cell growth, prolidase activity and collagen metabolism
After successful ascorbate and manganese treatment of a female patient with prolidase deficiency and iminodipeptiduria, we attempted to explain the mechanism of action of these drugsin vitro, using them preferent...
