121 Result(s)
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Life Sciences
Animal Genetics and Genomics
Evolutionary Biology
Plant Genetics and Genomics
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Article
Open AccessPrime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression
Most single nucleotide variants (SNVs) occur in noncoding sequence where millions of transcription factor binding sites (TFBS) reside. Here, a comparative analysis of CRISPR-mediated homology-directed repair (...
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Article
Open AccessGiotto: a toolbox for integrative analysis and visualization of spatial expression data
Spatial transcriptomic and proteomic technologies have provided new opportunities to investigate cells in their native microenvironment. Here we present Giotto, a comprehensive and open-source toolbox for spat...
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Article
A conservation-oriented SNP panel for Smallmouth Bass (Micropterus dolomieu), with emphasis on Interior Highlands lineages
The Smallmouth Bass (Micropterus dolomieu; SMB) is a widely distributed black bass species, but the southwestern edge of the species range within the Interior Highlands contains some of the most divergent ecotype...
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Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties
AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The full-length cDNAs of AGL19 in conventional Lonicera macranthoides (Lm-AGL19) and the mut...
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Open AccessFlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants
The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we presen...
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Identification of hybrid green peafowl using mitochondrial and nuclear markers
Green peafowl (Pavo muticus), a representative of the Southeast Asia Phasianidae, is assessed as globally endangered with populations that are declining. It is highly sensitive to human disturbance and habitat ch...
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Open AccessClonal tracing reveals diverse patterns of response to immune checkpoint blockade
Immune checkpoint blockade (ICB) therapy has improved patient survival in a variety of cancers, but only a minority of cancer patients respond. Multiple studies have sought to identify general biomarkers of IC...
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Open AccessAn integrated peach genome structural variation map uncovers genes associated with fruit traits
Genome structural variations (SVs) have been associated with key traits in a wide range of agronomically important species; however, SV profiles of peach and their functional impacts remain largely unexplored.
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Correction to: Complex patterns of genetic and morphological differentiation in the Smallmouth Bass subspecies (Micropterus dolomieu dolomieu and M. d. velox) of the Central Interior Highlands
In the original publication of the article, Table 2 was published incorrectly. The correct Table 2 is given in this Correction.
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Article
Complex patterns of genetic and morphological differentiation in the Smallmouth Bass subspecies (Micropterus dolomieu dolomieu and M. d. velox) of the Central Interior Highlands
Due to geologic processes and recent anthropogenic introductions, patterns of genetic and morphological diversity within the Smallmouth Bass (Micropterus dolomieu), which are endemic to the central and eastern Un...
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Article
Open AccesssgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine
We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among teste...
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Article
Open AccessIntegrative analyses of single-cell transcriptome and regulome using MAESTRO
We present Model-based AnalysEs of Transcriptome and RegulOme (MAESTRO), a comprehensive open-source computational workflow (http://github.com/liulab-dfci/MAESTRO)...
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Open AccessRepeat-induced point mutation in Neurospora crassa causes the highest known mutation rate and mutational burden of any cellular life
Repeat-induced point (RIP) mutation in Neurospora crassa degrades transposable elements by targeting repeats with C→T mutations. Whether RIP affects core genomic sequence in important ways is unknown.
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Open AccessSWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deamina...
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Open AccessModulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo
The CRISPR/Cas9 system is unable to edit all targetable genomic sites with full efficiency in vivo. We show that Cas9-mediated editing is more efficient in open chromatin regions than in closed chromatin regio...
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Article
The complete chloroplast genome of an endemic species of seed plants in China, Cleidiocarpon cavalerie (Malpighiales: Euphorbiaceae)
Cleidiocarpon cavaleriei (Euphorbiaceae) is native to China with high economic and ornamental value. In this study, we first characterized the complete chloroplast genome of C. cavaleriei based on next generation...
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Open AccessWhole genomes and transcriptomes reveal adaptation and domestication of pistachio
Pistachio (Pistacia vera), one of the most important commercial nut crops worldwide, is highly adaptable to abiotic stresses and is tolerant to drought and salt stresses.
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The complete chloroplast genome of a rare species Heritiera parvifolia Merr. (Malvales: Sterculiaceae)
The precious and endangered tree, Heritiera parvifolia Merr., is an excellent landscape and ornamental species endemic to China, but its populations are now very fragmented and in decline. It is currently on the ...
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The complete chloroplast genome sequence of the shrubby cinquefoil Dasiphora fruticosa (Rosales: Rosaceae)
The complete chloroplast genome of the shrubby cinquefoil Dasiphora fruticosa (Rosales: Rosaceae) has been assembled from the whole-genome Illumina sequencing data. The circular genome is 152,931 bp in size, and ...
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Open AccessDNA methylation footprints during soybean domestication and improvement
In addition to genetic variation, epigenetic variation plays an important role in determining various biological processes. The importance of natural genetic variation to crop domestication and improvement has...
