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Protocol
Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes
Acute myeloid leukemia (AML) is a disease caused by multiple distinct genomic events in the hematopoietic stem cell and progenitor compartment. To gain insight into the link between genetic mutations in AML an...
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Protocol
Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers
Multiplex immunoassays simultaneously measure multiple analytes in a single sample providing quantitative data via parallel analyses, which is especially suitable for serum biomarker verification and validatio...
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Protocol
Single-Stranded DNA Aptamers Against TNF and Their Potential Applications
Aptamers are short, single-stranded RNA or DNA sequences, which can bind to protein ligands with high affinity and specificity. Applications of aptamers are broad, ranging from drugs and drug delivery vehicles...
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Protocol
DNA Methylation in Atrial Fibrillation and Its Potential Role in Precision Medicine
Atrial fibrillation (AF), a common arrhythmia, can cause many serious consequences, including stroke and even death. The pathological mechanism of AF is very complicated. Epigenetic mechanisms, especially DNA ...
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Protocol
A Review on Cancer of Unknown Primary Origin: The Role of Molecular Biomarkers in the Identification of Unknown Primary Origin
The primary site cannot be found after clinical and pathological evaluation, which are called cancers of unknown primary origin (CUP). CUPs may resemble a specific primary tumor site which shares common clinic...
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Protocol
Airway Inflammation Biomarker for Precise Management of Neutrophil-Predominant COPD
Chronic obstructive pulmonary disease (COPD) course can be divided into stable stage and acute exacerbation. Deepen the understanding to the function and role of airway inflammatory cells in stable COPD is imp...
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Protocol
Nanopore Sequencing and Its Clinical Applications
Nanopore sequencing is a method for determining the order and modifications of DNA/RNA nucleotides by detecting the electric current variations when DNA/RNA oligonucleotides pass through the nanometer-sized ho...
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Protocol
Endoscopic Diagnosis and Treatment of Esophageal Squamous Cell Carcinoma
Esophageal squamous cell carcinoma (ESCC) is a deadly disease, partly because it is often diagnosed late in disease stage. An accurate early diagnosis by endoscopy could detect advanced carcinoma as well as cu...
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Protocol
Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells
A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial...
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Protocol
Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis
The survival from pancreatic cancer is poor because most patients are diagnosed after the cancer has metastasized. Liver is the most common site of pancreatic cancer metastasis. Orthotopic mouse models of live...
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Protocol
In Vitro Assay to Study CLL and Monocyte Interactions
Investigations focusing on CLL and microenvironment interaction allow understanding role of each component of the microenvironment. In vitro cell depletion assay we described here enables us to evaluate the de...
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Protocol
Genetically Engineered Plasma Membrane Nanovesicles for Cancer-Targeted Nanotheranostics
A series of ligand-targeted nanosystems have been rapidly exploited to selectively deliver drug molecules to desired cell populations. The conjugation of protein ligands to the nanoparticle (NP) surface endows...
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Protocol
Computational Methods for Subty** of Tumors and Their Applications for Deciphering Tumor Heterogeneity
With the rapid development of deep sequencing technologies, many programs are generating multi-platform genomic profiles (e.g., somatic mutation, DNA methylation, and gene expression) for a large number of tum...
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Protocol
Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells
Blood vessels in tumors contain chaotic branching structures and leaky vessel lumens, resulting in uneven supply of oxygen in the tumor microenvironment. High metabolic and proliferation rate of tumor cells fu...
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Protocol
Integration of Metabolomics and Transcriptomics to Identify Gene-Metabolite Relationships Specific to Phenotype
Metabolomics plays an increasingly large role in translational research, with metabolomics data being generated in large cohorts, alongside other omics data such as gene expression. With this in mind, we provi...
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Protocol
Computational Analysis of lncRNA Function in Cancer
Long noncoding RNAs (lncRNAs) have been shown to play crucial roles in cancer biology. With the help of computational analysis illustrated here, the joint effects of lncRNAs and clinical variables can be quant...
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Protocol
Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography–Tandem Mass Spectrometry
Mass spectrometry (MS)-based techniques have been finding utility as sensitive, high throughput metabolite analysis tools for complex biological samples. We describe here a nanoflow liquid chromatography–tande...
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Protocol
Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling
Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies and causes of cancer death. While the mortality rates from CRC have steadily declined, 50,000 individuals in the USA (and many times t...
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Protocol
Techniques for Evaluation of AR Transcriptional Output and Recruitment to DNA
Steroid receptors are ligand activated transcription factors whose promoter specificity is regulated by a broad set of coregulators and pioneer factors. Corepressors and coactivators determine receptors’ recru...
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Protocol
Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence
Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatograp...