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Article
Motivational determinants of motor performance: The hidden second task
The present study was designed to test facilitating and debilitating effects of motivational processes on motor behavior. It was predicted that motivational processes have a more pronounced effect on motor per...
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Chapter
Nearest Neighbour Analysis of the Photosystem I Subunits in Barley and Their Binding of Ferredoxin
Photosystem I (PS I) mediates electron transfer from plastocyanin to ferredoxin through the reaction center P700 and the five electron acceptors A0, A1, X, A and B (1). PS I preparations from barley contain the r...
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Chapter
Chloroplast Encoded Photosystem I Polypeptides of Barley
In higher plants, photochemical transfer of electrons from plastocyanin to ferredoxin is catalyzed by photosystem I (PS I), a membrane-bound protein complex binding the reaction center (P700), the photoreducib...
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Chapter
Investigation of Elasto-Mechanical Properties of Alginate by Ultrasound Methods
Alginate coated with poly-L-lysine (PLL) is one of the basic hydrogels for microencapsulation. Acting as “artificial cells”, some type of biological active material is encapsulated and implanted into the body,...
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Chapter
Lernen mit hypermedialen Lehr-/Lernsystemen — Zu Erwartungen, Erfahrungen und Möglichkeiten am Beispiel der Lernumgebung „WasserSportwissenschaft-online“
Das Projekt „WasserSportwissenschaft-online“ist eine hypermediale Lernumgebung, die als Ergänzung/Unterstützung der Präsenzlehre im Rahmen der Theorie-Praxisveranstaltungen zu den Wassersportarten Rudern, Kanu...
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Chapter
Robotikbaukästen als Lernumgebung in der universitären Informatikausbildung — ein Erfahrungsbericht
Um den sich ständig wandelnden inhaltlichen Ansprüchen des Informatik-Studiums gerecht zu werden, werden neue Lehr- und Lernszenarien eingesetzt, mit denen vielfältiges Wissen und Methodenkompetenz vermittelt,...
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Article
Polyomavirus tumorantigens have a profound effect on gene expression in mouse fibroblasts
Polyomavirus (Py) large and small tumorantigens together are competent to induce S phase in growth-arrested mouse fibroblasts. The capacity of the large tumorantigen to bind the pocket proteins, pRB, p130 and ...
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Chapter and Conference Paper
Mobile Autonomous Robots Play Soccer – An Intercultural Comparison of Different Approaches Due to Different Prerequisites
In the effort to meet the steadily changing demands of teaching computer science and computer engineering, new methods of learning and teaching are used by which multifarious knowledge and learning techniques ...
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Article
Cohesin mediates transcriptional insulation by CCCTC-binding factor
Cohesin complexes mediate sister-chromatid cohesion in dividing cells but may also contribute to gene regulation in postmitotic cells. How cohesin regulates gene expression is not known. Here we describe cohes...
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Article
The Suv39h–HP1 histone methylation pathway is dispensable for enrichment and protection of cohesin at centromeres in mammalian cells
Sister chromatids are physically connected by cohesin complexes. This sister chromatid cohesion is essential for the biorientation of chromosomes on the mitotic and meiotic spindle. In many species, cohesion b...
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Article
Open AccessGlutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-Aβ deposits in hippocampus via distinct cellular mechanisms
In the hippocampal formation of Alzheimer’s disease (AD) patients, both focal and diffuse deposits of Aβ peptides appear in a subregion- and layer-specific manner. Recently, pyroglutamate (pGlu or pE)-modified...
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Article
Open AccessDried blood spot UHPLC-MS/MS analysis of oseltamivir and oseltamivircarboxylate—a validated assay for the clinic
The neuraminidase inhibitor oseltamivir (Tamiflu®) is currently the first-line therapy for patients with influenza virus infection. Common analysis of the prodrug and its active metabolite oseltamivircarboxyla...
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Article
The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation
The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a Ra...
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Article
High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells
Automated fluorescence correlation spectroscopy data acquisition and analysis allows high throughput analysis of protein dynamics in cells.
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Article
Quantitative map** of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging
This protocol describes how to estimate and spatially resolve the concentration and copy number of fluorescently tagged proteins in live cells using fluorescence imaging and fluorescence correlation spectrosco...
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Article
Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing
This protocol provides a detailed workflow describing how to insert fluorescent markers into all alleles of a gene of interest using CRISPR–Cas9 technology, as well as how to generate and validate homozygous f...
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Article
Experimental and computational framework for a dynamic protein atlas of human cell division
Essential biological functions, such as mitosis, require tight coordination of hundreds of proteins in space and time. Localization, the timing of interactions and changes in cellular structure are all crucial...
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Article
A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent probes based on regular f...
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Article
Chemogenetic Control of Nanobodies
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engin...
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Article
Transient reprogramming of crop plants for agronomic performance
The development of a new crop variety is a time-consuming and costly process due to the reliance of plant breeding on gene shuffling to introduce desired genes into elite germplasm, followed by backcrossing. H...
