71 Result(s)

Article

Variant analysis of grain size related genes in the genus Sorghum

Grain size and weight are two of the most important determinants of crop yield. Key genes associated with the grain size and weight have been identified in major crops. However, studies on the genetic basis of...

Galaihalage K. S. Ananda, Sally L. Norton… in Genetic Resources and Crop Evolution (2023)

Article

A gene cluster in Ginkgo biloba encodes unique multifunctional cytochrome P450s that initiate ginkgolide biosynthesis

The ginkgo tree (Ginkgo biloba) is considered a living fossil due to its 200 million year’s history under morphological stasis. Its resilience is partly attributed to its unique set of specialized metabolites, in...

Victor Forman, Dan Luo, Fernando Geu-Flores, René Lemcke… in Nature Communications (2022)

Article

Tripterygium wilfordii cytochrome P450s catalyze the methyl shift and epoxidations in the biosynthesis of triptonide

The diterpenoid triepoxides triptolide and triptonide from Tripterygium wilfordii (thunder god wine) exhibit unique bioactivities with potential uses in disease treatment and as a non-hormonal male contraceptives...

Nikolaj Lervad Hansen, Louise Kjaerulff, Quinn Kalby Heck… in Nature Communications (2022)

Article

Transcript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism

Australian native species of sorghum contain negligible amounts of dhurrin in their leaves and the cyanogenesis process is regulated differently under water-stress in comparison to domesticated...

Galaihalage K. S. Ananda, Sally L. Norton, Cecilia Blomstedt, Agnelo Furtado… in Planta (2022)

Article

Regulation of dhurrin pathway gene expression during Sorghum bicolor development

Developmental and organ-specific expression of genes in dhurrin biosynthesis, bio-activation, and recycling offers dynamic metabolic responses optimizing growth and defence responses in Sorghum.

Roslyn M. Gleadow, Brian A. McKinley, Cecilia K. Blomstedt, Austin C. Lamb… in Planta (2021)

Article

Biased cytochrome P450-mediated metabolism via small-molecule ligands binding P450 oxidoreductase

Metabolic control is mediated by the dynamic assemblies and function of multiple redox enzymes. A key element in these assemblies, the P450 oxidoreductase (POR), donates electrons and selectively activates num...

Simon Bo Jensen, Sara Thodberg, Shaheena Parween, Matias E. Moses… in Nature Communications (2021)

Article

A flavin-dependent monooxygenase catalyzes the initial step in cyanogenic glycoside synthesis in ferns

Cyanogenic glycosides form part of a binary plant defense system that, upon catabolism, detonates a toxic hydrogen cyanide bomb. In seed plants, the initial step of cyanogenic glycoside biosynthesis—the conver...

Sara Thodberg, Mette Sørensen, Matteo Bellucci, Christoph Crocoll… in Communications Biology (2020)

Article

Nerylneryl diphosphate is the precursor of serrulatane, viscidane and cembrane-type diterpenoids in Eremophila species

Eremophila R.Br. (Scrophulariaceae) is a diverse genus of plants with species distributed across semi-arid and arid Australia. It is an ecologically important genus that also holds cultural significance for many ...

Oliver Gericke, Nikolaj Lervad Hansen, Gustav Blichfeldt Pedersen… in BMC Plant Biology (2020)

Article

Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses

Controlled turnover of proteins as mediated by the ubiquitin proteasome system (UPS) is an important element in plant defense against environmental and pathogen stresses. E3 ligases play a central role in subj...

Moon Young Ryu, Seok Keun Cho, Yourae Hong, **ho Kim, Jong Hum Kim… in BMC Genomics (2019)

Article

Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars

Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epihe...

Marcus Ehlert, Lea Møller Jagd, Ilka Braumann, Christoph Dockter… in Scientific Reports (2019)

Article

Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast

Forskolin is a high-value diterpenoid produced exclusively by the Lamiaceae plant Coleus forskohlii. Today forskolin is used pharmaceutically for its adenyl-cyclase activating properties. The limited availability...

Victor Forman, Niels Bjerg-Jensen, Jane D. Dyekjær… in Microbial Cell Factories (2018)

Article

Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans

The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather co...

Rasmus J. N. Frandsen, Paiman Khorsand-Jamal, Kenneth T. Kongstad… in Scientific Reports (2018)

Article

Direct observation of multiple conformational states in Cytochrome P450 oxidoreductase and their modulation by membrane environment and ionic strength

Cytochrome P450 oxidoreductase (POR) is the primary electron donor in eukaryotic cytochrome P450 (CYP) containing systems. A wealth of ensemble biophysical studies of Cytochrome P450 oxidoreductase (POR) has r...

Krutika Bavishi, Darui Li, Stine Eiersholt, Emma N. Hooley… in Scientific Reports (2018)

Article

Label-free Raman hyperspectral imaging analysis localizes the cyanogenic glucoside dhurrin to the cytoplasm in sorghum cells

Localisation of metabolites in sorghum coleoptiles using Raman hyperspectral imaging analysis was compared in wild type plants and mutants that lack cyanogenic glucosides. This novel method allows high spatial...

Philip Heraud, Max F. Cowan, Katarzyna Maria Marzec… in Scientific Reports (2018)

Article

Characterization of a membrane-bound C-glucosyltransferase responsible for carminic acid biosynthesis in Dactylopius coccus Costa

Carminic acid, a glucosylated anthraquinone found in scale insects like Dactylopius coccus, has since ancient times been used as a red colorant in various applications. Here we show that a membrane-bound C-glucos...

Rubini Kannangara, Lina Siukstaite, Jonas Borch-Jensen… in Nature Communications (2017)

Article

Assembly of Dynamic P450-Mediated Metabolons—Order Versus Chaos

We provide an overview of the current knowledge on cytochrome P450-mediated metabolism organized as metabolons and factors that facilitate their stabilization. Essential parameters will be discussed including ...

Jean-Etienne Bassard, Birger Lindberg Møller… in Current Molecular Biology Reports (2017)

Article

Erratum to: Spatial analysis of root hemiparasitic shrubs and their hosts: a search for spatial signatures of above- and below-ground interactions

Bjørn Dueholm, David Bruce, Philip Weinstein, Susan Semple… in Plant Ecology (2017)