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Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells

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Recombinant Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2762))

Abstract

Hantaviruses, are rodent-borne viruses found worldwide that are transmitted to humans through inhalation of contaminated excreta. They can cause a renal or a pulmonary syndrome, depending on the virus, and no effective treatment is currently available for either of these diseases. Hantaviral particles are covered by a protein lattice composed of two glycoproteins (Gn and Gc) that mediate adsorption to target cells and fusion with endosomal membranes, making them prime targets for neutralizing antibodies. Here we present the methodology to produce soluble recombinant glycoproteins in different conformations, either alone or as a stabilized Gn/Gc complex, using stably transfected Drosophila S2 cells.

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Authors and Affiliations

  1. G5 Structural Biology of Infectious Diseases, Institut Pasteur, Université Paris Cité, Paris, France

    Annalisa Meola & Pablo Guardado-Calvo

Authors
  1. Annalisa Meola
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  2. Pablo Guardado-Calvo
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Corresponding author

Correspondence to Pablo Guardado-Calvo .

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Editors and Affiliations

  1. Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA

    Steven B. Bradfute

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Meola, A., Guardado-Calvo, P. (2024). Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3666-4_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3665-7

  • Online ISBN: 978-1-0716-3666-4

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